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Titolo:
Oxygen dependency of monoamine oxidase activity in the intact lung
Autore:
Audi, SH; Dawson, CA; Ahlf, SB; Roerig, DL;
Indirizzi:
Zablocki Vet Adm Med Ctr, Res Serv 151, Dept Vet Affairs, Milwaukee, WI 53295 USA Zablocki Vet Adm Med Ctr Milwaukee WI USA 53295 , Milwaukee, WI 53295 USA Marquette Univ, Dept Biomed Engn, Milwaukee, WI 53201 USA Marquette Univ Milwaukee WI USA 53201 iomed Engn, Milwaukee, WI 53201 USA Med Coll Wisconsin, Dept Anesthesiol, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 hesiol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Dept Pharmacol Toxicol, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 oxicol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 hysiol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Dept Pulm & Crit Care Med, Milwaukee, WI 53226 USA MedColl Wisconsin Milwaukee WI USA 53226 re Med, Milwaukee, WI 53226 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: L969 - L981
SICI:
1040-0605(200110)281:4<L969:ODOMOA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE AMINE OXIDASE; PERFUSED RABBIT LUNG; HYDROGEN-PEROXIDE GENERATION; RAT-LIVER; TOXICITY; DAMAGE; CELLS; DISPOSITION; DEAMINATION; INHIBITION;
Keywords:
multiple indicator dilution; mathematical modeling; endothelial cells; pargyline; semicarbazide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Audi, SH Zablocki Vet Adm Med Ctr, Res Serv 151, Dept Vet Affairs, 5000 W Natl Ave,Milwaukee, WI 53295 USA Zablocki Vet Adm Med Ctr 5000 W Natl Ave Milwaukee WI USA 53295 A
Citazione:
S.H. Audi et al., "Oxygen dependency of monoamine oxidase activity in the intact lung", AM J P-LUNG, 281(4), 2001, pp. L969-L981

Abstract

Hydrogen peroxide generated by monoamine oxidase (MAO)-mediated deamination of biogenic amines has been implicated in cell signaling and oxidative injury. Because the pulmonary endothelium is a site of metabolism of monoamines present in the venous return, this brings into question a role for MAO in hyperoxic lung injury. The objective of this study was to evaluate the O-2 dependency of the MAO reaction in the lung. To this end, we measured the pulmonary venous effluent concentrations of the MAO substrate [C-14]phenylethylamine and its metabolite [C-14] phenylacetic acid after the bolus injection of either phenylethylamine or phenylacetic acid into the pulmonary artery of perfused rabbit lungs over a range Of Po-2 values from 16 to 518 Torr. The apparent Michaelis constant for O-2 was similar to 18 muM, which is more than an order of magnitude less that measured for purified MAO. The results suggest a minimal influence of high O-2 on MAO activity in the normallung and demonstrate the importance of measuring reaction kinetics in the intact organ.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 10:36:28