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Titolo:
Proteins and lipids define the diffusional field of nitric oxide
Autore:
Porterfield, DM; Laskin, JD; Jung, SK; Malchow, RP; Billack, B; Smith, PJS; Heck, DE;
Indirizzi:
Rutgers State Univ, Environm & Occupat Hlth Sci Inst, Piscataway, NJ 08854USA Rutgers State Univ Piscataway NJ USA 08854 Inst, Piscataway, NJ 08854USA Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJ USA 08854 scataway, NJ 08854 USA Marine Biol Lab, BioCurrents Res Ctr, Woods Hole, MA 02543 USA Marine BiolLab Woods Hole MA USA 02543 Res Ctr, Woods Hole, MA 02543 USA Univ Illinois, Dept Biol, Chicago, IL 60607 USA Univ Illinois Chicago IL USA 60607 nois, Dept Biol, Chicago, IL 60607 USA Univ Illinois, Dept Ophthalmol & Visual Sci, Chicago, IL 60607 USA Univ Illinois Chicago IL USA 60607 ol & Visual Sci, Chicago, IL 60607 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: L904 - L912
SICI:
1040-0605(200110)281:4<L904:PALDTD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-NITROSYLATION; POTENTIAL ROLE; SERUM-ALBUMIN; INHIBITION; MECHANISM; SYNTHASE; CELLS; HEPATOTOXICITY; NITROSOTHIOLS; MACROPHAGES;
Keywords:
nitric oxide flux; nitric oxide synthase; self-referencing electrode;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Heck, DE Rutgers State Univ, Environm & Occupat Hlth Sci Inst, 170 Frelinghuysen Rd, Piscataway, NJ 08854 USA Rutgers State Univ 170 Frelinghuysen RdPiscataway NJ USA 08854 A
Citazione:
D.M. Porterfield et al., "Proteins and lipids define the diffusional field of nitric oxide", AM J P-LUNG, 281(4), 2001, pp. L904-L912

Abstract

Nitric oxide (NO) fluxes released from the surface of individual activatedmacrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. NO fluxes could be detectedat distances from the cells of 100-500 mum. The initial flux and the distance from the cells at which NO could be detected were directly related to the number of cells in the immediate vicinity of the probe releasing NO. Thus, whereas NO fluxes of similar to1 pmol.cm(-2).s(-1) were measured from individual macrophages, aggregates composed of groups of cells varying in number from 18 to 48 cells produced NO fluxes of between similar to4 and 10 pmol.cm(-2).s(-1). NO fluxes required the presence Of L-arginine. Signals were significantly reduced by the addition of hemoglobin and by N-nitro-L-arginine methyl ester. NO fluxes were greatest when the sensor was placed immediately adjacent to cell membranes and declined as the distance from the cell increased. The NO signal was markedly reduced in the presence of the protein albumin but not by either oxidized or reduced glutathione. A reduction in the NO signal was also noted after the addition of lipid micelles to theculture medium. These results demonstrate that NO can be detected at significant distances from the cell of origin. In addition, both proteins and lipids strongly influence the net movement of free NO from macrophages. This suggests that these tissue components play an important role in regulating the biological activity of NO.

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Documento generato il 06/04/20 alle ore 08:27:53