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Titolo:
A reversibly immortalized human hepatocyte cell line as a source of hepatocyte-based biological support
Autore:
Kobayashi, N; Westerman, KA; Tanaka, N; Fox, IJ; Leboulch, P;
Indirizzi:
Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol Surg Transplantat & Surg Oncol, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 & Surg Oncol, Okayama 7008558, Japan MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA MIT Cambridge MA USA02139 iv Hlth Sci & Technol, Cambridge, MA 02139 USA Genetix Pharmaceut, Cambridge, MA USA Genetix Pharmaceut Cambridge MA USA enetix Pharmaceut, Cambridge, MA USA Univ Nebraska, Med Ctr, Dept Surg, Omaha, NE USA Univ Nebraska Omaha NE USA v Nebraska, Med Ctr, Dept Surg, Omaha, NE USA
Titolo Testata:
ADDICTION BIOLOGY
fascicolo: 4, volume: 6, anno: 2001,
pagine: 293 - 300
SICI:
1355-6215(200109)6:4<293:ARIHHC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LIVER-FAILURE; LARGE TUMOR-ANTIGEN; GENE-TRANSFER; RAT HEPATOCYTES; BONE-MARROW; TRANSPLANTATION; THERAPY; MODEL; MICE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol Surg Transplantat & Surg Oncol, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "A reversibly immortalized human hepatocyte cell line as a source of hepatocyte-based biological support", ADDICT BIOL, 6(4), 2001, pp. 293-300

Abstract

The application of hepatocyte transplantation (HTX) is increasingly envisioned for temporary metabolic support during acute liver failure and provision of specific liver functions in inherited liver-based metabolic diseases. Compared with whole liver transplantation, HTX is a technically simple procedure and hepatocytes can be cryopreserved for future use. A major limitation of this form of therapy in humans is the worldwide shortage of human livers for isolating an adequate number of transplantable human hepatocyes when needed. Fur ther more, the numbers of donor livers available for hepatocyte isolation is limited by competition for their use in whole organ transplantation. Considering the cost of hepatocyte isolation and the need for immediate preparation of consistent and functional cells, it is unlikely thathuman hepatocytes can be obtained on such a scale to treat a large number of patients with falling liver functions. The utilization of xenogenic hepatocytes will result in additional concerns regarding transmission of infectious pathogens and immunological and physiological incompatibilities between animals and humans. An attractive alter native to primary human hepatocytes is the use of tightly regulated human hepatocyte cell lines. Such cell lines can provide the advantages of unlimited availability, sterility and uniformity. We describe here methods for creating transplantable human hepatocyte cell lines using currently available cell cultures and gene transfer technology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:07:27