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Titolo:
TNF-alpha-induced cell death in feline immunodeficiency virus-infected cells is mediated by the caspase cascade
Autore:
Mizuno, T; Goto, Y; Baba, K; Masuda, K; Ohno, K; Tsujimoto, H;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 rnal Med, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 287, anno: 2001,
pagine: 446 - 455
SICI:
0042-6822(20010901)287:2<446:TCDIFI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; PERIPHERAL-BLOOD LYMPHOCYTES; NF-KAPPA-B; T-CELLS; (FIV)-ASSOCIATED LYMPHOMA; IMMUNE DYSFUNCTION; INDUCED APOPTOSIS; CATS; ACTIVATION; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Tsujimoto, H Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 yo 1138657, Japan
Citazione:
T. Mizuno et al., "TNF-alpha-induced cell death in feline immunodeficiency virus-infected cells is mediated by the caspase cascade", VIROLOGY, 287(2), 2001, pp. 446-455

Abstract

TNF-alpha induced apoptosis in a feline fibroblastic cell line (CRFK) infected with FIV but not in its uninfected control. In this study, to understand the molecular basis of the different susceptibilities to TNF-alpha between FIV-Infected and uninfected cells, we examined the expression of TNF receptors and the activation of the caspase and NF-kappaB pathways, Expressionlevels of TNFR I and TNFR II mRNAs were similar between uninfected and FIV-infected CRFK cells, To understand the role of caspases in TNF-alpha -induced apoptosis, we examined the effect of three different classes of caspaseinhibitors, Z-VAD-FMK, Ac-YVAD-CMK, and Z-DEVD-FMK, on the TNF-alpha -induced apoptosis in FIV-infected cells. Pretreatment with each of these caspase inhibitors protected FIV-infected CRFK cells from TNF-alpha -induced celldeath. Moreover, one of the caspase substrates, poly(ADP-ribose) polymerase, was shown to be cleaved after TNF-alpha treatment in FIV-infected CRFK cells but not in uninfected CRFK cells. Electrophoretic mobility shift assayusing an NF-kappaB motif oligonucleotide and promoter assay using an NF-kappaB luciferase reporter construct indicated that TNF-alpha treatment had induced activation of NF-kappaB in both FIV-infected and uninfected CRFK cells. The present study indicates that TNF-alpha -induced apoptosis in FIV-Infected CRFK cells Is mediated by the activation of the caspase cascade, butnot by either upregulation of TNF receptor or inhibition of NF-kappaB. (C)2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:56:58