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Titolo:
Molecular evolution of Mycobacterium tuberculosis: phylogenetic reconstruction of clonal expansion
Autore:
Warren, RM; Richardson, M; Sampson, SL; van der Spuy, GD; Bourn, W; Hauman, JH; Heersma, H; Hide, W; Beyers, N; van Helden, PD;
Indirizzi:
Univ Stellenbosch, Dept Med Biochem, MRC, Ctr Cellular & Mol Biol, ZA-7505Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa Univ Stellenbosch, Dept Paediat & Child Hlth, ZA-7505 Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Bellville, South Africa Univ Western Cape Bellville South Africa ZA-7535 Bellville, South Africa Natl Inst Publ Hlth & Environm Protect, NL-3720 BA Bilthoven, Netherlands Natl Inst Publ Hlth & Environm Protect Bilthoven Netherlands NL-3720 BA
Titolo Testata:
TUBERCULOSIS
fascicolo: 4, volume: 81, anno: 2001,
pagine: 291 - 302
SICI:
1472-9792(2001)81:4<291:MEOMTP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-INCIDENCE COMMUNITY; NEW-YORK-CITY; ESCHERICHIA-COLI; GENOME SEQUENCE; COMPLEX STRAINS; IS6110; EPIDEMIOLOGY; MARKERS; ELEMENT; TRANSPOSITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: van Helden, PD Univ Stellenbosch, Dept Med Biochem, MRC, Ctr Cellular & Mol Biol, POB 19063, ZA-7505 Tygerberg, South Africa Univ Stellenbosch POB 19063 Tygerberg South Africa ZA-7505
Citazione:
R.M. Warren et al., "Molecular evolution of Mycobacterium tuberculosis: phylogenetic reconstruction of clonal expansion", TUBERCULOSI, 81(4), 2001, pp. 291-302

Abstract

Setting: M. tuberculosis isolates were collected from patients attending health clinics in a high incidence urban community and in a low incidence rural setting in South Africa. Objective: To reconstruct the evolutionary history of a group of closely related M. tuberculosis isolates using IS6110, DRr and MTB484(1) restrictionfragment length polymorphism (RFLP) data. Design: Mycobacterium tuberculosis isolates containing an average of ten IS6110 elements, with a similarity index of greater than or equal to 65% were genotypically classified by DNA fingerprinting using the IS6110 derived probes IS-3 ' and IS-5 ' as well as the DRr and MTB484(1) probes, in combination with Pvull or Hinfl endonuclease digestion. These RFLP data were subjected to phylogenetic analysis using both genetic distance and parsimony algorithms. Results: Phylogenetic analysis predicted the existence of two independently evolving lineages, possibly evolving from a common ancestral strain. The topology of the phylogenetic tree was supported by comprehensive bootstrapping and the specific partitioning of DNA methylation phenotypes. The observed difference in the branch lengths of the two lineages may suggest differential evolutionary rates. Isolates collected from different geographical regions demonstrate independent evolution, suggesting that it is highly unlikely that strains have been recently transmitted between the two regions. The number of evolutionary events identified in this strain family differs significantly from that of previously characterized strain families, implyingthat evolutionary rate may be strain family dependent. Conclusion: Based on this analysis we propose that the algorithm used to calculate recent epidemiological events should be revised to incorporate theevolutionary characteristics of individual strain families, thereby enhancing the accuracy of molecular epidemiological calculations. (C) 2001 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:31:33