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Titolo:
The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells
Autore:
Sachana, M; Flaskos, J; Alexaki, E; Glynn, P; Hargreaves, AJ;
Indirizzi:
Nottingham Trent Univ, Dept Life Sci, Nottingham NG11 8NS, England Nottingham Trent Univ Nottingham England NG11 8NS gham NG11 8NS, England Aristotelian Univ Thessaloniki, Fac Vet Med, Toxicol & Biochem Lab, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006 niki, Greece Univ Leicester, MRC, Toxicol Res Unit, Leicester LE11 5RH, Leics, England Univ Leicester Leicester Leics England LE11 5RH LE11 5RH, Leics, England
Titolo Testata:
TOXICOLOGY IN VITRO
fascicolo: 4-5, volume: 15, anno: 2001,
pagine: 369 - 372
SICI:
0887-2333(200108/10)15:4-5<369:TTOCTD>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROPATHY TARGET ESTERASE; IN-VITRO; PC12 CELLS; NEUROTOXICITY; PROTEINS; VIVO;
Keywords:
differentiating neuroblastoma; neurite outgrowth; neurofilament heavy chain; heat shock protein; neuropathy target esterase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Hargreaves, AJ Nottingham Trent Univ, Dept Life Sci, Clifton Lane, Nottingham NG11 8NS, England Nottingham Trent Univ Clifton Lane Nottingham England NG11 8NS
Citazione:
M. Sachana et al., "The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells", TOX VITRO, 15(4-5), 2001, pp. 369-372

Abstract

The aim of this work was to study the effects of chlorpyrifos (CPF) on theoutgrowth of axons by differentiating mouse N2a neuroblastoma cells. This was achieved by morphological, Western blotting and enzymatic analyses of cells induced to differentiate in the presence and absence of CPF added either at the same time (co-differentiation) or 16 h after (post-differentiation) the induction of cell differentiation. The outgrowth of axon-like processes was impaired following 4 or 8 h exposure to CPF in both co- and post-differentiation experiments. Western blotting analysis revealed reduced levels of neurofilament heavy chain (NF-H) following 8 h of exposure but no significant effect at 4 h under both co- and post-differentiation conditions. By contrast, levels of the heat shock protein HSP-70 were raised at both time points, but only in co-differentiation experiments. Neuropathy target esterase (NTE) activity was lower than controls following 4 or 8 h of exposureunder co-differentiation conditions, but not under any post-differentiation conditions. The results suggest that the inhibition of axon production and maintenance by CPF in differentiating N2a cells may involve multiple targets, which are different under co- and post-differentiation conditions. (C)2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:40:53