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Titolo:
Flow cytometry detection of platelet procoagulant activity and microparticles in patients with unstable angina treated by percutaneous coronary angioplasty and stent implantation
Autore:
Vidal, C; Spaulding, C; Picard, F; Schaison, F; Melle, J; Weber, S; Fontenay-Roupie, M;
Indirizzi:
Hop Cochin, Cent Hematol Lab, F-75014 Paris, France Hop Cochin Paris France F-75014 Cent Hematol Lab, F-75014 Paris, France Hop Cochin, Serv Cardiol, F-75014 Paris, France Hop Cochin Paris France F-75014 hin, Serv Cardiol, F-75014 Paris, France
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 3, volume: 86, anno: 2001,
pagine: 784 - 790
SICI:
0340-6245(200109)86:3<784:FCDOPP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYOCARDIAL-INFARCTION; ACTIVATED PLATELETS; MEMBRANE-GLYCOPROTEINS; AMINOPHOSPHOLIPID EXPOSURE; SCOTT SYNDROME; P-SELECTIN; ANNEXIN-V; IN-VIVO; SURFACE; ARTERY;
Keywords:
human blood platelets; flow cytometry; annexin V; microvesiculation; unstable angina;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Fontenay-Roupie, M Hop Cochin, Cent Hematol Lab, 27 Rue Faubourg St Jacques, F-75014 Paris, France Hop Cochin 27 Rue Faubourg St Jacques Paris France F-75014
Citazione:
C. Vidal et al., "Flow cytometry detection of platelet procoagulant activity and microparticles in patients with unstable angina treated by percutaneous coronary angioplasty and stent implantation", THROMB HAEM, 86(3), 2001, pp. 784-790

Abstract

Platelet activation is known to participate to the pathogenesis of acute coronary syndromes. Aminophospholipid exposure and microparticles shedding are hallmarks of full platelet activation and may account for the dissemination of prothrombotic seats. Using flow, cytometry analysis of annexin V binding to externalized aminophospholipids, we followed platelet procoagulant activity (PPA) and platelet microparticles (PMP) shedding in venous and coronary whole blood samples from 30 patients with unstable angina before and after percutaneous coronary angioplasty (PTCA) and stent implantation. Baseline values of PPA and PMP were significantly more elevated in patients than in control subjects (p < 0.005). PMP percentage was significantly higher in coronary than in venous blood, and in coronary blood of patients With proximal instead of mid/distal lesions of coronary arteries. No enhancement of platelet reactivity to TRAP and collagen was induced by procedure. Whereas activated GpIIb-IIIa and P-selectin expression decreased 24 h and 48 h after procedure, PPA and PMP remained as elevated as before. Thus, flow cytometry is a reliable method for detection of fully activated platelets in whole blood samples. Annexin V binding analysis demonstrates the persistance of in vivo platelet activation, despite the use of antiaggregating agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:37:40