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Titolo:
Regional distribution of endothelin-1 and endothelin converting enzyme-1 in porcine endotoxemia
Autore:
Magder, S; Javeshghani, D; Cernacek, P; Giaid, A;
Indirizzi:
McGill Univ, Dept Med, Div Crit Care, Montreal, PQ, Canada McGill Univ Montreal PQ Canada Med, Div Crit Care, Montreal, PQ, Canada McGill Univ, Dept Physiol, Div Crit Care, Montreal, PQ, Canada McGill Univ Montreal PQ Canada siol, Div Crit Care, Montreal, PQ, Canada McGill Univ, Ctr Hlth, Royal Victoria Hosp, Montreal, PQ, Canada McGill Univ Montreal PQ Canada Royal Victoria Hosp, Montreal, PQ, Canada
Titolo Testata:
SHOCK
fascicolo: 4, volume: 16, anno: 2001,
pagine: 320 - 325
SICI:
1073-2322(200110)16:4<320:RDOEAE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULMONARY-HYPERTENSION; PLASMA ENDOTHELIN-1; RECEPTOR ANTAGONIST; MESSENGER-RNA; NEUROPEPTIDE-Y; NITRIC-OXIDE; SHOCK; EXPRESSION; SEPSIS; BOSENTAN;
Keywords:
sepsis; hyperdynamic state; hypotension;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Magder, S McGill Univ, Royal Victoria Hosp, Crit Care Div, 687 Pine Ave W,Montreal,PQ H3A 1A1, Canada McGill Univ 687 Pine Ave W Montreal PQ Canada H3A 1A1 A1, Canada
Citazione:
S. Magder et al., "Regional distribution of endothelin-1 and endothelin converting enzyme-1 in porcine endotoxemia", SHOCK, 16(4), 2001, pp. 320-325

Abstract

Endothelin-1 (ET-1) levels are markedly increased in sepsis. Since ET-1 isprimarily transcriptionally regulated, there should be a corresponding increase in pre-pro-endothelin-1 (ppET-1). Our objective was to determine whether ppET-1 is increased in pigs with a low systemic vascular resistance. Wealso examined the distribution of ET-1 and the regulation of endothelin-converting enzyme 1 (ECE-1), the rate limiting enzyme in ET-1 production. We anesthetized and ventilated 16 pigs. We measured arterial, pulmonary, and central venous pressures, as well as cardiac output. ET-1 was measured by radioimmunoassay in plasma and in multiple tissues. We infused 20 mug/kg of endotoxin over 2 h and then sacrificed the animals. ppET-1 and ECE-1 mRNA were assessed by Northern analysis. We performed immunohistochemistry for theassessment of tissue ET-1 and ECE-1. The systemic vascular resistance roseat 30 min, but fell by 120 min. Plasma ET-1 more than doubled by 2 h. However, there was no change in the concentration of ET-1 in any tissue except in the pulmonary artery. By immunohistochemistry, there was also no change in ET-1 in aorta, vena cava, heart, lung, liver, and kidney. Distribution of ECE-1 followed that of ET-1 on immunohistochemistry. There was a significant increase in ppET-1 mRNA in liver, kidney papillae, and vena cava, and atendency for an increase in other tissues. This was paralleled by an increase in ECE-1 mRNA. In conclusion, the amount of ECE-1 mRNA and protein parallel those of ET-1. Endotoxemia is associated with a marked increase in plasma ET-1 and an increase in ppET-1 and ECE-1 mRNA in multiple tissues; however, there was no significant change in tissue ET-1 except in the pulmonaryartery. The rise in plasma levels without a change in tissue levels suggests a greater release into the vasculature in sepsis than under normal conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 22:33:08