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Titolo:
Mesenteric lymph from rats subjected to trauma-hemorrhagic shock are injurious to rat pulmonary microvascular endothelial cells as well as human umbilical vein endothelial cells
Autore:
Deitch, EA; Adams, CA; Lu, Q; Xu, DZ;
Indirizzi:
UMDNJ, New Jersey Med Sch, Dept Surg, Newark, NJ 07103 USA UMDNJ Newark NJ USA 07103 Jersey Med Sch, Dept Surg, Newark, NJ 07103 USA
Titolo Testata:
SHOCK
fascicolo: 4, volume: 16, anno: 2001,
pagine: 290 - 293
SICI:
1073-2322(200110)16:4<290:MLFRST>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LUNG INJURY; BACTERIAL TRANSLOCATION; GUT; NEUTROPHILS; ISCHEMIA; ORGAN;
Keywords:
cytotoxicity; cell monolayer permeability; gut-induced distant organ injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Deitch, EA UMDNJ, New Jersey Med Sch, Dept Surg, Room G506,185 S Orange Ave, Newark, NJ 07103 USA UMDNJ Room G506,185 S Orange Ave Newark NJ USA 07103 07103 USA
Citazione:
E.A. Deitch et al., "Mesenteric lymph from rats subjected to trauma-hemorrhagic shock are injurious to rat pulmonary microvascular endothelial cells as well as human umbilical vein endothelial cells", SHOCK, 16(4), 2001, pp. 290-293

Abstract

Previously, we have documented that gut-derived lymph from rats subjected to trauma/hemorrhagic shock (T/HS) is injurious to human umbilical vein endothelial cells (HUVEC). To verify these findings in an all rat systems, theability of T/HS lymph to increase rat pulmonary microvascular endothelial cell (RPMVEC) monolayer permeability and kill RPMVEC was compared with thatobserved with HUVECs. RPMVEcs isolated from male rats or HUVECs were grownin 24-well plates for the cytotoxicity assays or on permeable filters in atwo-chamber system for permeability assays. Mesenteric lymph was collectedfrom male rats subjected to trauma (laparotomy) plus hemorrhagic shock (T/HS group) or to a laparotomy plus sham-shock (T/SS group). The T/HS group had their mean arterial pressure decreased to 30 mmHg and kept there for 90 min. Lymph samples centrifuged to remove the cellular component were incubated with the RPMVECs or HUVECs at a 10% concentration. Neither T/SS lymph nor post-T/HS portal vein plasma was toxic to or increased the permeability of the RPMVECs or HUVECs. The pattern of cytotoxicity observed in the HUVECs incubated with T/HS mesenteric lymph was similar to that observed in the RPMVECs, as reflected by trypan blue dye exclusion, with more than 95% of the HUVECs and RPMVECs being killed after a 16-h incubation with T/HS mesenteric lymph. However, at earlier time points the amount of LDH released fromthe HUVEC cells incubated with T/HS lymph was greater than that observed with the PRMVEC, although trypan blue dye exclusion was similar. Similarly, incubation with 10% T/HS lymph increased the permeability of both HUVEC andRPMVEC monolayers more than 2-fold, even with an incubation period as short as 1 h. In conclusion, these results provide further evidence that T/HS lymph, but not T/SS lymph or post-T/HS portal vein plasma, is injurious to endothelial cells and that RPMVECs are as susceptible to injury as HUVECs. Additionally, these studies support the emerging concept that gut-induced distant organ injury is mediated by factors contained in mesenteric lymph.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:19:04