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Titolo:
Comparison of STIM and particle backscattering spectrometry mass determination for quantitative microanalysis of cultured cells
Autore:
Deves, G; Ortega, R;
Indirizzi:
Univ Bordeaux 1, UMR 5084 CNRS, LCNAB, F-33175 Gradignan, France Univ Bordeaux 1 Gradignan France F-33175 CNAB, F-33175 Gradignan, France
Titolo Testata:
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
, volume: 181, anno: 2001,
pagine: 460 - 464
SICI:
0168-583X(200107)181:<460:COSAPB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
OVARIAN ADENOCARCINOMA CELLS; CISPLATIN-RESISTANT; MICROPROBE ANALYSIS; BIOLOGICAL SAMPLES; NUCLEAR MICROSCOPY; PLATINUM; DAMAGE;
Keywords:
STIM; RBS; nuclear microprobe; quantitative microanalysis; PIXE; mass loss; cell thickness;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Engineering, Computing & Technology
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ortega, R Univ Bordeaux 1, UMR 5084 CNRS, LCNAB, BP 120 Haut Vigneau, F-33175 Gradignan, France Univ Bordeaux 1 BP 120 Haut Vigneau Gradignan FranceF-33175 ce
Citazione:
G. Deves e R. Ortega, "Comparison of STIM and particle backscattering spectrometry mass determination for quantitative microanalysis of cultured cells", NUCL INST B, 181, 2001, pp. 460-464

Abstract

In biological sample microanalysis, a mass-normalisation method is commonly used as a quantitative index of elemental concentrations determined by particle-induced X-ray emission (PIXE). The organic mass can either be determined using particle backscattering spectrometry (BS) or scanning transmission ion microscopy (STIM). However, the accuracy of quantitative microanalysis in samples such as cultured cells is affected by beam-induced loss of organic mass during analysis. The aim of this paper is to compare mass measurements determined by particle BS or by STIM. In order to calibrate STIM andBS analyses, we measured by both techniques the thickness of standard foils of polycarbonate (3 and 6 mum), Mylar (R) (4 mum), Kapton (R) (7.5 mum) and Nylon (R) (15 mum), as well as biological samples of mono-layered cultured cells. Non-damaging STIM analysis of samples before PIXE irradiation is certainly one of the most accurate ways to determine the sample mass, however, this requires strong experimental handling. On the other hand, BS performed simultaneously to PIXE is the simplest method to determine the local mass in polymer foils, but appears less accurate in the case of cultured cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:17:59