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Titolo:
Anterograde and retrograde transport of active extracellular signal-related kinase 1 (ERK1) in the ligated rat sciatic nerve
Autore:
Reynolds, AJ; Hendry, IA; Bartlett, SE;
Indirizzi:
Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Canberra, ACT2601, Australia Australian Natl Univ Canberra ACT Australia 2601 erra, ACT2601, Australia Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld, Australia Univ Queensland St Lucia Qld Australia armacol, St Lucia, Qld, Australia
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 105, anno: 2001,
pagine: 761 - 771
SICI:
0306-4522(2001)105:3<761:AARTOA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; AXONAL-TRANSPORT; PHOSPHATIDYLINOSITOL 3-KINASE; SYMPATHETIC NEURONS; TYROSINE KINASE; TRKA; SURVIVAL; CELL; NGF; NEUROTROPHINS;
Keywords:
axonal transport; mitogen-activated protein kinase; neurons; serine/threonine protein kinase B; signal transduction; nerve injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Bartlett, SE Univ Queensland, Dept Physiol & Pharmacol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 4072, Australia
Citazione:
A.J. Reynolds et al., "Anterograde and retrograde transport of active extracellular signal-related kinase 1 (ERK1) in the ligated rat sciatic nerve", NEUROSCIENC, 105(3), 2001, pp. 761-771

Abstract

Neurons are one of the most polarized cells and often the nerve terminals may be located long distances from the cell body, thus signal transduction in neurons unlike other cells may need to be conducted over large distances. The mitogen-activated protein/extracellular signal-regulated kinases (MAPkinases or ERKs) regulate a diverse array of functions and in neurons, theERK signalling pathways appear to have an important role in activity-dependent regulation of neuronal function. Using the ligated rat sciatic nerve as an experimental model we previously showed that the ERK1/2, MAP/ERK kinase (MEK1/2) and the p110 catalytic subunit of P13-kinase are transported in the rat sciatic nerve. We have extended these findings to determine if these proteins are transported in the active state using antibodies that specifically detect the active form of ERK1/2, MEK1/2 and AKT which is activated downstream of P13-kinase. We show significant accumulation of active ERK1 on the proximal and distal sides of a nerve ligation after 16 h. Active ERK2also appeared to be accumulating at the ligature however this did not reach statistical significance. In contrast there was not any significant accumulation of active MEK1/2 or active AKT. A component of both active ERK1 andactive ERK2 is present in between the two ligations suggesting they are also present in the surrounding Schwann cells and are activated in response to nerve injury. Taken together our results suggest that a component of the accumulation ofactive ERK1 on the distal and proximal side of the nerve ligations resultsfrom transport in the anterograde and retrograde direction in the rat sciatic nerve. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:08:06