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Titolo:
Upregulation of gap junction connexin 32 with epileptiform activity in theisolated mouse hippocampus
Autore:
Li, J; Shen, H; Naus, CCG; Zhang, L; Carlen, PL;
Indirizzi:
Univ Hlth Network, Toronto Western Res Inst, Div Cellular & Mol Biol, Bloorview Epilepsy Program, Toronto, ON M5T 2S8, Canada Univ Hlth Network Toronto ON Canada M5T 2S8 , Toronto, ON M5T 2S8, Canada Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, Canada Univ Western Ontario London ON Canada N6A 5C1 London, ON N6A 5C1, Canada
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 105, anno: 2001,
pagine: 589 - 598
SICI:
0306-4522(2001)105:3<589:UOGJC3>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONAL CARCINOMA-CELLS; ADULT CEREBRAL-CORTEX; DIFFERENTIAL EXPRESSION; SEIZURE DISORDER; GENE-EXPRESSION; SMOOTH-MUSCLE; CALCIUM WAVES; IN-VITRO; C-FOS; BRAIN;
Keywords:
connexins; bicuculline; carbenoxolone; epileptiform activity; transcription factors; mouse isolated hippocampus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Carlen, PL Toronto Western Hosp, 399 Bathurst St,McL 12-413, Toronto, ON M5T 2S8, Canada Toronto Western Hosp 399 Bathurst St,McL 12-413 Toronto ON Canada M5T 2S8
Citazione:
J. Li et al., "Upregulation of gap junction connexin 32 with epileptiform activity in theisolated mouse hippocampus", NEUROSCIENC, 105(3), 2001, pp. 589-598

Abstract

Gap junctions which serve as intercellular channels providing direct cytoplasmic continuity and ionic current flow between adjacent cells, are constituted by connexin proteins. Using an in vitro model of bicuculline-induced epileptiform activity, we asked whether increased connexin levels occur during epileptiform activity in the intact whole hippocampus, freshly isolatedfrom young (15-day-old) mouse brain. Exposure to bicuculline (10 muM), for2-10 h, induced persistent changes in electrical activities that included enhanced spontaneous field activity (4 h), an epileptiform response to single electrical stimulation (6 h), and spontaneous epileptiform activity (6 h). These electrophysiological changes were not reversed by up to 60 nun perfusion with normal artificial cerebrospinal fluid, but were greatly depressed by the gap junction uncoupler, carbenoxolone (120 muM, 10 min). Data from RNase protection assay and immunoblotting showed that among several detected gap junctions, only connexin 32 was affected. After 2-6 h exposure to bicuculline, the connexin 32 mRNA expression was upregulated to 2-3-fold control (P < 0.01), and its protein level was significantly elevated the following 6 h (P < 0.01), at which time electrophysiologically measured evidenceof clearly epileptiform activity was apparent. In addition, the transcription factor, c-fos protein, but not the cAMP response element-binding protein, was also found to be increased at the early stage of bicuculline exposure (2 h) compared to control (P < 0.05). Thus we have found that exposing the acutely isolated hippocampus to bicuculline, induced increased c-fos protein, followed by increased connexin 32 transcript and protein, and concurrently, persistent epileptiform activity that was depressed by carbenoxolone. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 06/07/20 alle ore 05:37:04