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Titolo:
Prolonged epileptiform bursting induced by 0-Mg2+ in rat hippocampal slices depends on gap junctional coupling
Autore:
Kohling, R; Gladwell, SJ; Bracci, E; Vreugdenhil, M; Jefferys, JGR;
Indirizzi:
Univ Birmingham, Sch Med, Div Neurosci Neurophysiol, Birmingham B15 2TT, WMidlands, England Univ Birmingham Birmingham W Midlands England B15 2TT WMidlands, England Univ Munster, Inst Physiol, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 t Physiol, D-48149 Munster, Germany
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 105, anno: 2001,
pagine: 579 - 587
SICI:
0306-4522(2001)105:3<579:PEBIB0>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY SYNAPTIC-INTERACTIONS; SYNCHRONIZED AFTER-DISCHARGES; EPILEPTOGENESIS IN-VITRO; CA1 PYRAMIDAL CELLS; SPREADING DEPRESSION; GABA(A) RECEPTORS; NEURONAL-ACTIVITY; CALCIUM; OSCILLATIONS; INTERNEURONS;
Keywords:
gap junctions; seizure; ictogenesis; hippocampus; magnesium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Jefferys, JGR Univ Birmingham, Sch Med, Div Neurosci Neurophysiol, VincentDr, Birmingham B15 2TT, W Midlands, England Univ Birmingham Vincent Dr Birmingham W Midlands England B15 2TT
Citazione:
R. Kohling et al., "Prolonged epileptiform bursting induced by 0-Mg2+ in rat hippocampal slices depends on gap junctional coupling", NEUROSCIENC, 105(3), 2001, pp. 579-587

Abstract

The transition from brief interictal to prolonged seizure, or 'ictal', activity is a crucial event in epilepsy. In vitro slice models can mimic many phenomena observed in the electroencephalogram of patients, including transition from interictal to ictaform or seizure-like activity. In field potential recordings, three discharge types can be distinguished: (1) primary discharges making up the typical interictal burst, (2) secondary bursts, lasting several hundred milliseconds, and (3) tertiary discharges lasting for seconds, constituting the ictal series of bursts. The roles of chemical synapses in these classes of burst have been explored in detail. Here we test the hypothesis that gap junctions are necessary for the generation of secondary bursts. In rat hippocampal slices, epileptiform activity was induced by exposure to 0-Mg2+. Epileptiform discharges started in the CA3 subfield, and generally consisted of primary discharges followed by 4-13 secondary bursts. Three drugs that block gap junctions, halothane (5-10 mM), carbenoxolone (100 muM) and octanol (0.2-1.0 mM), abolished the secondary discharges, but left the primary bursts intact. The gap junction opener trimethylamine (10 mM) reversibly induced secondary and tertiary discharges. None of these agents altered intrinsic or synaptic properties of CA3 pyramidal cells at the doses used. Surgically isolating the CA3 subfield made secondary discharges disappear, and trimethylamine under these conditions was able to restore them. We conclude that gap junctions can contribute to the prolongation of epileptiform discharges. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 24/01/20 alle ore 12:29:24