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Titolo:
The SH2 domain-containing 5-phosphatase SHIP2 is expressed in the germinallayers of embryo and adult mouse brain: Increased expression in N-CAM-deficient mice
Autore:
Muraille, E; Dassesse, D; Vanderwinden, JM; Cremer, H; Rogister, B; Erneux, C; Schiffmann, SN;
Indirizzi:
Free Univ Brussels, Lab Neurophysiol CP601, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 601, B-1070 Brussels, Belgium Free Univ Brussels, IRIBHN, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 BHN, B-1070 Brussels, Belgium Univ Mediterranee, Lab Genet & Physiol Dev, IBDM, CNRS,INSERM, F-13288 Marseille, France Univ Mediterranee Marseille France F-13288 RM, F-13288 Marseille, France Univ Liege, Ctr Cellular & Mol Neurobiol, Liege, Belgium Univ Liege Liege Belgium , Ctr Cellular & Mol Neurobiol, Liege, Belgium
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 105, anno: 2001,
pagine: 1019 - 1030
SICI:
0306-4522(2001)105:4<1019:TSD5SI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-GAMMA-RIIB; INOSITOL POLYPHOSPHATE 5-PHOSPHATASE; MESSENGER-RNA EXPRESSION; SUBVENTRICULAR ZONE; MAMMALIAN BRAIN; NERVOUS-SYSTEM; PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE; TYROSINE PHOSPHORYLATION; PHOSPHATASE SHIP; FACTOR-RECEPTOR;
Keywords:
phosphatidylinositol metabolism; brain development; ventricular zone; rostral migratory stream; N-CAM; neural stem cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Schiffmann, SN Free Univ Brussels, Lab Neurophysiol CP601, Campus Erasme,Bldg C,808 RouteLennik, B-1070 Brussels, Belgium Free Univ Brussels Campus Erasme,Bldg C,808 Route Lennik Brussels Belgium B-1070
Citazione:
E. Muraille et al., "The SH2 domain-containing 5-phosphatase SHIP2 is expressed in the germinallayers of embryo and adult mouse brain: Increased expression in N-CAM-deficient mice", NEUROSCIENC, 105(4), 2001, pp. 1019-1030

Abstract

The germinative ventricular zone of embryonic brain contains neural lineage progenitor cells that give rise to neurons, astrocytes and oligodendrocytes. The ability to generate neurons persists at adulthood in restricted brain areas. During development, many growth factors exert their effects by interacting with tyrosine kinase receptors and activate the phosphatidylinositol 3-kinase and the Ras/MAP kinase pathways. By its ability to modulate these pathways, the recently identified Src homology 2 domain-containing inositol polyphosphate 5-phosphatase 2, SHIP2, has the potential to regulate neuronal development. Using in situ hybridization technique with multiple synthetic oligonucleotides, we demonstrated that SHIP2 mRNA was highly expressed in the ventricular zone at early embryonic stages and subventricular zones at latter stages of brain and spinal cord and in the sympathetic chain. No significant expression was seen in differentiated fields. This restricted expression was maintained from embryonic day 11.5 to birth. In the periphery, large expression was detected in muscle and kidney and moderate expression in thyroid, pituitary gland, digestive system and bone. In the adult brain, SHIP2 was mainly restricted in structures containing neural stem cells such as the anterior subventricular zone, the rostral migratory stream and the olfactory tubercle. SHIP2 was also detected in the choroid plexuses and the granular layer of the cerebellum. The specificity of SHIP2 expression in neural stem cells was further demonstrated by (i) the dramatic increase in SHIP2 mRNA signal in neural cell adhesion molecule (N-CAM)-deficient mice, which present an accumulation of progenitor cells in the anterior subventricular zone and the rostral migratory stream, (ii) the abundant expression of 160-kDa SHIP2 by western blotting in proliferating neurospheres in culture and its downregulation in non-proliferating differentiated neurospheres. In conclusion, the close correlation between the pattern of SHIP2 expression in the brain and the proliferative and early differentiative events suggests that the phosphatase SHIP2 may have important roles in neural development. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:10:05