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Titolo:
Checkpoint activation in response to double-strand breaks requires the Mre11/Rad50/Xrs2 complex
Autore:
Grenon, M; Gilbert, C; Lowndes, NF;
Indirizzi:
Imperial Canc Res Fund, Clare Hall Labs, CDC Lab, Potters Bar EN6 3LD, Herts, England Imperial Canc Res Fund Potters Bar Herts England EN6 3LD , Herts, England Natl Univ Ireland Univ Coll Galway, Dept Biochem, Galway, Ireland Natl Univ Ireland Univ Coll Galway Galway Ireland chem, Galway, Ireland
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 9, volume: 3, anno: 2001,
pagine: 844 - 847
SICI:
1465-7392(200109)3:9<844:CAIRTD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGE CHECKPOINT; LYMPHOBLASTOID CELL-LINES; BUDDING YEAST; MEC1-DEPENDENT PHOSPHORYLATION; ATAXIA-TELANGIECTASIA; PROTEIN COMPLEX; CYCLE; REPAIR; RAD53; INTERACTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Lowndes, NF Imperial Canc Res Fund, Clare Hall Labs, CDC Lab, Blanche Lane, Potters Bar EN6 3LD, Herts, England Imperial Canc Res Fund Blanche Lane Potters Bar Herts England EN6 3LD
Citazione:
M. Grenon et al., "Checkpoint activation in response to double-strand breaks requires the Mre11/Rad50/Xrs2 complex", NAT CELL BI, 3(9), 2001, pp. 844-847

Abstract

Studies of human Nijmegen breakage syndrome (NBS) cells have led to the proposal that the Mre11/Rad50/NBS1 complex, which is involved in the repair of DNA double-strand breaks (DSBs), might also function in activating the DNA damage checkpoint pathways after DSBs occur(1,2). We have studied the role of the homologous budding yeast complex, Mre11/Rad50/Xrs2, in checkpoint activation in response to DSB-inducing agents. Here we show that this complex is required for phosphorylation and activation of the Rad53 and Chk1 checkpoint kinases specifically in response to DSBs. Consistent with defectiveRad53 activation, we observed defective cell-cycle delays after induction of DSBs in the absence of Mrell. Furthermore, after gamma -irradiation phosphorylation of Rad9, which is an early event in checkpoint activation, is also dependent on Mre11. All three components of the Mre11/Rad50/Xrs2 complex are required for activation of Rad53, however, the Ku80, Rad51 or Rad52 proteins, which are also involved in DSB repair, are not. Thus, the integrity of the Mre11/Rad50/Xrs2 complex is specifically required for checkpoint activation after the formation of DSBs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:23:52