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Titolo:
Organisation of the cytoskeleton during in vitro maturation of horse oocytes
Autore:
Tremoleda, JL; Schoevers, EJ; Stout, TAE; Colenbrander, B; Bevers, MM;
Indirizzi:
Univ Utrecht, Fac Vet Med, Dept Equine Sci, Sect Reprod, NL-3584 CM Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CM 3584 CM Utrecht, Netherlands Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, Utrecht, Netherlands Univ Utrecht Utrecht Netherlands t Farm Anim Hlth, Utrecht, Netherlands
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 60, anno: 2001,
pagine: 260 - 269
SICI:
1040-452X(200110)60:2<260:OOTCDI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL GRANULE EXOCYTOSIS; IN-VITRO; EQUINE OOCYTES; MOUSE OOCYTES; MEIOTIC MATURATION; PORCINE OOCYTES; BOVINE OOCYTES; FOLLICLE SIZE; CHROMATIN CONFIGURATION; MICROTUBULE DYNAMICS;
Keywords:
microtubules; microfilaments; chromatin; equine; oocyte; meiotic maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Tremoleda, JL Univ Utrecht, Fac Vet Med, Dept Equine Sci, Sect Reprod, Yalelaan 12, NL-3584 CM Utrecht, Netherlands Univ Utrecht Yalelaan 12 UtrechtNetherlands NL-3584 CM nds
Citazione:
J.L. Tremoleda et al., "Organisation of the cytoskeleton during in vitro maturation of horse oocytes", MOL REPROD, 60(2), 2001, pp. 260-269

Abstract

Meiotic maturation of mammalian oocytes is a complex process during which microfilaments and microtubules provide the framework for chromosomal reorganisation and cell division. The aim of this study was to use fluorescence and confocal laser scanning microscopy to examine changes in the distribution of these important cytoskeletal elements and their relationship to chromatin configuration during the maturation of horse oocytes in vitro. Oocyteswere cultured in M199 supplemented with pFSH and eLH and, at 0, 12, 24, and 36 hr after the onset of culture, they were fixed for immunocytochemistryand stained with markers for microtubules. (a monoclonal anti-alpha -tubulin antibody), microfilaments (AlexaFluor 488 Phalloidin) and DNA (TO-PRO3). At the germinal vesicle stage, oocyte chromatin was amorphous and poorly condensed and the microfilaments and microtubules were distributed relatively evenly throughout the ooplasm. After germinal vesicle breakdown, the microtubules were aggregated around the now condensed chromosomes and the microfilaments had become concentrated within the oocyte cortex. During metaphase I, microtubules were detected only in the meiotic spindle, as elongated asters encompassing the aligned chromosomes, and, as maturation progressed through anaphase-I and telophase-I, the spindle assumed a more eccentric position and gradually rotated to assist in the separation of the homologous chromosomes and in the subsequent formation of the first polar body. During metaphase II, the meiotic spindle was a symmetrical, barrel-shaped structure with two poles and with the chromosomes aligned along its midline. At this stage, microtubules were found intermingled with chromatin within the polar body and, although, the bulk of the microfilaments remained within the oocyte cortex, a rich domain was found overlying the spindle. Thus, during the in vitro maturation of horse oocytes both the microfilament and microtubular elements of the cytoskeleton were seen to reorganise dramatically in afashion that appeared to enable chromosomal alignment and segregation. Mol. Reprod. Dev. 60: 260-269, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:35:17