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Titolo:
Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast
Autore:
Kosco, KA; Pearson, CG; Maddox, PS; Wang, PJJ; Adams, IR; Salmon, ED; Bloom, K; Huffaker, TC;
Indirizzi:
Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 t Mol Biol & Genet, Ithaca, NY 14853 USA Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Biol, Chapel Hill, NC 27599 USA MRC, Mol Biol Lab, Cambridge CB2 2QH, England MRC Cambridge England CB2 2QH , Mol Biol Lab, Cambridge CB2 2QH, England
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 9, volume: 12, anno: 2001,
pagine: 2870 - 2880
SICI:
1059-1524(200109)12:9<2870:COMDBS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SISTER-CHROMATID SEPARATION; SACCHAROMYCES-CEREVISIAE; MITOTIC SPINDLE; BUDDING YEAST; POLE BODY; PRECOCIOUS SEPARATION; ESSENTIAL COMPONENT; CELL CORTEX; PROTEIN; TUBULIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Huffaker, TC Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 Genet, Ithaca, NY 14853 USA
Citazione:
K.A. Kosco et al., "Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast", MOL BIOL CE, 12(9), 2001, pp. 2870-2880

Abstract

Stu2p is a member of a conserved family of microtubule-binding proteins and an essential protein in yeast. Here, we report the first in vivo analysisof microtubule dynamics in cells lacking a member of this protein family. For these studies, we have used a conditional Stu2p depletion strain expressing alpha -tubulin fused to green fluorescent protein. Depletion of Stu2p leads to fewer and less dynamic cytoplasmic microtubules in both GI and preanaphase cells. The reduction in cytoplasmic microtubule dynamics is due primarily to decreases in both the catastrophe and rescue frequencies and an increase in the fraction of time microtubules spend pausing. These changes have significant consequences for the cell because they impede the ability of cytoplasmic microtubules to orient the spindle. In addition, recovery offluorescence after photobleaching indicates that kinetochore microtubules are no longer dynamic in the absence of Stu2p. This deficiency is correlated with a failure to properly align chromosomes at metaphase. Overall, we provide evidence that Stu2p promotes the dynamics of microtubule plus-ends invivo and that these dynamics are critical for microtubule interactions with kinetochores and cortical sites in the cytoplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:16:38