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Titolo:
Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA
Autore:
Tang, S; He, WJ; Xu, H; Liu, WY; Ruan, KC;
Indirizzi:
Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 200031, Peoples R China
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 223, anno: 2001,
pagine: 117 - 121
SICI:
0300-8177(200107)223:1-2<117:EEF2CB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-II; ADP-RIBOSYLATION; DIPHTHERIA-TOXIN; PROTEIN; TRANSLOCATION; MECHANISM;
Keywords:
ADP-ribosylation; eukaryotic elongation factor 2 (eEF2); ribosomal RNA; sarcin/ricin domain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, WY Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China Chinese Acad Sci 320 Yue Yang Rd Shanghai Peoples R China 200031
Citazione:
S. Tang et al., "Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA", MOL C BIOCH, 223(1-2), 2001, pp. 117-121

Abstract

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to P site by binding to the ribosome. In this work, the complex formation of rat liver eEF2 with a synthetic oligoribonucleotide (SRD RNA) that mimics sarcin/ricin domain of rat 28S ribosomal RNA is invested in vitro. Purified eEF2 can specifically bind SRD RNA to form a stable complex. tRNA competes with SRD RNA in binding to eEF2 in a less extent. Pretreatment of eEF2 with GDP or ADP-ribosylation of eEF2 by diphtheria toxin can obviously reduce the ability of eEF2 to form the complex with the synthetic oligoribonucleotide. These results indicate that eEF2 is likely to bind directly to the sarcin/ricin domain of 28S ribosomal RNA in the process of protein synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:07:22