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Titolo:
Subcellular distribution of cytokeratin and vimentin in malignant rhabdoidtumor: Three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence
Autore:
Itakura, E; Tamiya, S; Morita, K; Shiratsuchi, H; Kinoshita, Y; Oshiro, Y; Oda, Y; Ohta, S; Furue, M; Tsuneyoshi, M;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ, Grad Sch Med Sci, Dept Dermatol, Higashi Ku, Fukuoka 8128582,Japan Kyushu Univ Fukuoka Japan 8128582 tol, Higashi Ku, Fukuoka 8128582,Japan Shiga Univ Med Sci, Dept Pediat, Shiga, Japan Shiga Univ Med Sci Shiga Japan Univ Med Sci, Dept Pediat, Shiga, Japan
Titolo Testata:
MODERN PATHOLOGY
fascicolo: 9, volume: 14, anno: 2001,
pagine: 854 - 861
SICI:
0893-3952(200109)14:9<854:SDOCAV>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATIONAL-WILMS-TUMOR; INTERMEDIATE FILAMENTS; CULTURED-CELLS; SOFT-TISSUES; KIDNEY; EXPRESSION; GENE; HSNF5/INI1; PROTEINS; SARCOMAS;
Keywords:
confocal laser scanning microscopy; cytokeratin; intermediate filament; malignant rhabdoid tumor; vimentin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuneyoshi, M Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Higashi Ku,Maidashi 3-1-1, Fukuoka 8128582, Japan Kyushu Univ Maidashi 3-1-1 FukuokaJapan 8128582 582, Japan
Citazione:
E. Itakura et al., "Subcellular distribution of cytokeratin and vimentin in malignant rhabdoidtumor: Three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence", MOD PATHOL, 14(9), 2001, pp. 854-861

Abstract

Malignant rhabdoid tumor (MRT) is a highly aggressive neoplasm that mostlyoccurs in childhood, characterized histologically by rhabdoid. cells as shown by eosinophilic intracytoplasmic inclusions. Although it is known that rhabdoid cells co-express cytokeratin (CK) and vimentin, the distribution patterns of these two kinds of intermediate filaments and structural relationship between them are still not known. We investigated the subcellular distribution of CKs 8 and 18 and vimentin in MRT cell lines (Tm87-16, STM91-01, TTC549, and TC289) using confocal laser scanning microscopy and double immunofluorescence, in addition to ultrastructural examination. Vimentin was diffusely expressed in the cytoplasm of MRT cells, focally forming a filamentous network. In contrast, CKs 8 and 18 were partially expressed hi the cytoplasm of MRT cells, forming globules or a few vague agglomerates. Three-dimensional images in TC289 cells revealed distinct distribution patterns ofcytokeratin and vimentin, showing agglomerates of cytokeratins within the vimentin filament network. We conclude that these globules and agglomeratesof CKs 8 and 18 correspond with the characteristic ultrastructural finding, showing cytoplasmic bundles of intermediate filaments concentrated in whorled arrays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:51:34