Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Tachyzoite-specific isoform of Toxoplasma gondii lactate dehydrogenase is the target antigen of a murine CD4(+) T-cell clone
Autore:
Reichmann, G; Dlugonska, H; Hiszczynska-Sawicka, E; Fischer, HG;
Indirizzi:
Univ Dusseldorf, Inst Med Microbiol & Virol, D-40225 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany Univ Lodz, Inst Microbiol & Immunol, PL-90237 Lodz, Poland Univ Lodz Lodz Poland PL-90237 icrobiol & Immunol, PL-90237 Lodz, Poland Gdansk Tech Univ, Dept Microbiol, PL-80952 Gdansk, Poland Gdansk Tech Univ Gdansk Poland PL-80952 crobiol, PL-80952 Gdansk, Poland
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 10, volume: 3, anno: 2001,
pagine: 779 - 787
SICI:
1286-4579(200108)3:10<779:TIOTGL>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION CLONING; LYMPHOCYTES-T; INFECTION; PROTEIN; RESISTANCE; ELECTROPHORESIS; MACROPHAGES; DEPLETION; IMMUNITY; CD4+;
Keywords:
Toxoplasma gondii; 2-D T-cell blot; stage conversion; lactate dehydrogenase; T-cell epitope;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, HG Univ Dusseldorf, Inst Med Microbiol & Virol, Univ Str 1 Geb 22-21, D-40225Dusseldorf, Germany Univ Dusseldorf Univ Str 1 Geb 22-21 Dusseldorf Germany D-40225
Citazione:
G. Reichmann et al., "Tachyzoite-specific isoform of Toxoplasma gondii lactate dehydrogenase is the target antigen of a murine CD4(+) T-cell clone", MICROBES IN, 3(10), 2001, pp. 779-787

Abstract

In two-dimensionally separated Toxoplasma gondii lysate, mouse Th1 clone 3Tx15 detects two proteins of apparent molecular weight 40 000 and pI of 5.8and 5.9. Microsequencing of peptide fragments from tryptic digestion of one of these proteins yielded partial sequences of T. gondii lactate dehydrogenase (LDH)1. As shown by Western blot, toxoplasmic LDH co-migrates in two-dimensional get electrophoresis with both T-cell antigenic proteins. With synthetic peptides spanning the complete primary structure of T gondii LDH1,the T-cell epitope was mapped to a nine amino acid partial sequence which exhibits a motif for binding to I-E-k the class II restriction element of antigen recognition by clone 3Tx15. From the two known isoforms of T gondii LDH, clone 3Tx15 specifically recognises tachyzoite LDH1, but not bradyzoite LDH2, as shown with the corresponding epitope peptides and recombinant proteins. Antigen-presenting cells infected with live bradyzoites stimulate 3Tx15 T cells, while killed bradyzoites provide no antigenic stimulus. This finding implies that a transformation into the tachyzoite stage occurs in cells challenged with bradyzoites. Although LDH1 represents one major constituent of the tachyzoite proteome, the protein does not seem to be immunogenic in T gondii infection of mice. This is evident from the lack of serum anti-LDH immunoreactivity and the failure of adoptively transferred 3Tx15 T cells to protect against lethal challenge. In conclusion, a T-cell-stimulatory Toxoplasma antigen is identified by means of a novel, high-resolution T-cell blot technique the clone's antigenic fine specificity allowing detection of parasite-stage conversion. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 10:42:19