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Titolo:
Temperature-programmed non-aqueous electrochromatographic separation of retinyl esters
Autore:
Roed, L; Lundanes, E; Greibrokk, T;
Indirizzi:
Univ Oslo, Dept Chem, N-0315 Oslo, Norway Univ Oslo Oslo Norway N-0315Univ Oslo, Dept Chem, N-0315 Oslo, Norway
Titolo Testata:
JOURNAL OF SEPARATION SCIENCE
fascicolo: 6, volume: 24, anno: 2001,
pagine: 435 - 443
SICI:
1615-9314(200107)24:6<435:TNESOR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; CONTINUOUS-BED COLUMNS; RIGID POLYMER MONOLITHS; GEL BONDED OCTADECYLSILICA; CAPILLARY ELECTROCHROMATOGRAPHY; MASS-SPECTROMETRY; SILICA COLUMNS; NONAQUEOUS ELECTROCHROMATOGRAPHY; ELECTROOSMOTIC FLOW; STATIONARY-PHASE;
Keywords:
temperature programming; non-aqueous; electrochromatography; retinyl esters; continuous bed;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Roed, L Univ Oslo, Dept Chem, POB 1033 Blindern, N-0315 Oslo, Norway Univ Oslo POB 1033 Blindern Oslo Norway N-0315 -0315 Oslo, Norway
Citazione:
L. Roed et al., "Temperature-programmed non-aqueous electrochromatographic separation of retinyl esters", J SEP SCI, 24(6), 2001, pp. 435-443

Abstract

An in-house assembled electrochromatograph was used for temperature-controlled non-aqueous electrochromatography. Reversed-phase separations of retinyl esters were performed on continuous bed columns. The continuous bed columns were prepared by sol-gel bonding of C-30 material in 180 mum internal diameter fused silica capillaries. The mobile phase consisted of 2.5 mM lithium acetate in N,N-dimethylformamide-acetonitrile-methanol (2 + 7 + 1, v/v). The use of temperature programming increased the resolution of earlier-eluting compounds as well as improved the peak shape and decreased the retention time of later-eluting compounds. Separations of retinyl esters (all-trans-retinyl acetate, palmitate, heptadecanoate, stearate, oleate, and linoleate) were completed in 12 minutes. The within-day and between-day variations of retention times of all-trans-retinyl palmitate were <0.6% (n = 6) relative standard deviation (RSD) and 2.3% (n = 3) RSD, respectively. Further, the within-day and between-day variations of peak areas were <2.7% (n = 6) RSD, and 3.4% (n = 3) RSD, respectively. The column examined was stable formore than two weeks of continuous injections of standard solutions and real samples. Lipid extracts of bearded seal (Erignathus barbatus) liver were analyzed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 22:27:34