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Titolo:
Toxic effects of mitomycin-c on cultured ciliary process cells and trabecular meshwork cells
Autore:
Hong, SJ; Wu, KY; Wang, HZ; Lai, YH;
Indirizzi:
Kaohsiung Med Univ, Dept Pharmacol, Kaohsiung, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan , Dept Pharmacol, Kaohsiung, Taiwan
Titolo Testata:
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
fascicolo: 4, volume: 17, anno: 2001,
pagine: 331 - 342
SICI:
1080-7683(200108)17:4<331:TEOMOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORNEAL ENDOTHELIUM; HYPOTONY; APOPTOSIS; GLAUCOMA; SURGERY; RABBIT; CONTRACTILITY; EPITHELIUM; PRESSURE; TIMOLOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hong, SJ Kaohsiung Med Univ, Dept Pharmacol, 100 Shih Chuan 1st Rd, Kaohsiung, Taiwan Kaohsiung Med Univ 100 Shih Chuan 1st Rd Kaohsiung Taiwan aiwan
Citazione:
S.J. Hong et al., "Toxic effects of mitomycin-c on cultured ciliary process cells and trabecular meshwork cells", J OCUL PH T, 17(4), 2001, pp. 331-342

Abstract

Mitomycin-C has recently become an adjunct medication for inhibition of fibroblast proliferation in glaucoma filtering procedures. Prolonged postoperative ocular hypotony has been a frequent complication of trabeculectomy with mitomycin-C. In order to characterize the hypotony mechanism, we compared the toxic effects of mitomycin-C on cultured rabbit ciliary process cellsand trabecular meshwork cells. The results indicate that mitomycin-C has amore marked effect on ciliary process cells on H-3-thymidine uptake than on trabecular meshwork cells at concentrations ranging from 10(-1) to 10(-5)mg/ml after 3-, 5- and 60-min treatment, respectively. The living cells after mitomycin-C treatment were estimated with MTT assay that was converted tetrazolium dye of living cells only into insoluble purple formazan crystals within mitochondria. In the presence of mitomycin-C for 3, 5, and 60 min,the cellular MTT values in ciliary process cells were more decreased than in trabecular meshwork cells. Depolarization of the trabecular meshwork cells with 50 mM KCl led to an increase in intracellular calcium concentration, whereas application of mitomycin-C at 10(-3) mg/ml resulted in decrease of KCl-induced intracellular calcium increase. Mitomycin-C (10(-3) mg/ml) decreased cAMP concentration in ciliary process cells following 3- and 5-min treatment; however, it did not significantly affect the cellular cAMP concentration after only a 1-min exposure. Mitomycin-induced marked ladder pattern of DNA fragmentation was observed in ciliary process tissues after treatment with 10(-1)mg/ml of mitomycin-C for 3 and 5 min. However, the DNA pattern in trabecular meshwork tissues was not obviously affected by mitomycin-C. These findings from our results indicate that mitomycin-induced ocular hypotony may result from damage to both ciliary process and trabecular meshwork tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:36:32