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Titolo:
HIV-1 Nef co-localizes with the astrocyte-specific cytoskeleton protein GFAP in persistently nef-expressing human astrocytes
Autore:
Kohleisen, B; Hutzler, P; Shumay, E; Ovod, V; Erfle, V;
Indirizzi:
GSF Natl Res Ctr Environm & Hlth, Inst Mol Virol, D-85764 Neuherberg, Germany GSF Natl Res Ctr Environm & Hlth Neuherberg Germany D-85764 erg, Germany Inst Pathol, D-85764 Neuherberg, Germany Inst Pathol Neuherberg Germany D-85764 thol, D-85764 Neuherberg, Germany Bavarian Nord, D-82152 Martinsried, Germany Bavarian Nord Martinsried Germany D-82152 , D-82152 Martinsried, Germany Univ Tampere, Inst Med Technol, Tampere 33520, Finland Univ Tampere Tampere Finland 33520 t Med Technol, Tampere 33520, Finland
Titolo Testata:
JOURNAL OF NEUROVIROLOGY
fascicolo: 1, volume: 7, anno: 2001,
pagine: 52 - 55
SICI:
1355-0284(200102)7:1<52:HNCWTA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; DOWN-REGULATION; MYRISTOYLATION; ACTIVATION; CELLS; CD4; ASSOCIATION; INFECTION; DOMAINS; KINASE;
Keywords:
HIV-1; Nef; GFAP; co-localisation; astrocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Erfle, V GSF Natl Res Ctr Environm & Hlth, Inst Mol Virol, Ingolstaedter Landstr 1,D-85764 Neuherberg, Germany GSF Natl Res Ctr Environm & Hlth Ingolstaedter Landstr 1 Neuherberg Germany D-85764
Citazione:
B. Kohleisen et al., "HIV-1 Nef co-localizes with the astrocyte-specific cytoskeleton protein GFAP in persistently nef-expressing human astrocytes", J NEUROVIRO, 7(1), 2001, pp. 52-55

Abstract

In T-cells HIV-1 Nef exerts various functions and interacts with actin. Inastrocytes interaction of Nef xvith cellular proteins is poorly understood. Therefore, human astrocytic cell clones stably transfected with nef-genesderived from HIV-1 Bru and its myristoylation-defective TH-variant were investigated by confocal laser scanning microscopy for expression of Nef and cytoskeleton proteins actin and GFAP, a marker for activated astrocytes. Myristoylated Nef was detected in cytoplasm, Golgi and plasmamembrane, while non-myristoylated Nef was exclusively cytoplasmic. Nef co-localised with GFAP in the perinuclear region of astrocytes. In contrast, Nef did not interact with actin filaments in human astrocytes. Nef/GFAP interaction could contribute to changes in morphology and activation state of astrocytes shown previously which are both critical for development of astrogliosis in HIV-1 infected brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:18:46