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Titolo:
Long-term maintenance of channel distribution in a central pattern generator neuron by neuromodulatory inputs revealed by decentralization in organ culture
Autore:
Mizrahi, A; Dickinson, PS; Kloppenburg, P; Fenelon, V; Baro, DJ; Harris-Warrick, RM; Meyrand, P; Simmers, J;
Indirizzi:
CNRS, F-33405 Talence, France CNRS Talence France F-33405CNRS, F-33405 Talence, France Cornell Univ, Dept Neurobiol & Behav, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 Neurobiol & Behav, Ithaca, NY 14853 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 18, volume: 21, anno: 2001,
pagine: 7331 - 7339
SICI:
0270-6474(20010915)21:18<7331:LMOCDI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOBSTER STOMATOGASTRIC GANGLION; TRANSIENT POTASSIUM CURRENT; SHAL GENE-EXPRESSION; DOPAMINE MODULATION; NEUROMUSCULAR-JUNCTION; SKELETAL-MUSCLE; PYLORIC NETWORK; SINGLE NEURONS; VOLTAGE CLAMP; PHASE-SHIFTS;
Keywords:
crustacean; stomatogastric ganglion; motor network; identified pyloric neuron; organ culture; decentralization; shal K+ channel; shal immunodetection; voltage clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Simmers, J Univ Bordeaux 1, Lab Neurobiol Reseaux, Ave Fac, F-33405 Talence, France Univ Bordeaux 1 Ave Fac Talence France F-33405 Talence, France
Citazione:
A. Mizrahi et al., "Long-term maintenance of channel distribution in a central pattern generator neuron by neuromodulatory inputs revealed by decentralization in organ culture", J NEUROSC, 21(18), 2001, pp. 7331-7339

Abstract

Organotypic cultures of the lobster (Homarus gammarus) stomatogastric nervous system (STNS) were used to assess changes in membrane properties of neurons of the pyloric motor pattern-generating network in the long-term absence of neuromodulatory inputs to the stomatogastric ganglion (STG). Specifically, we investigated decentralization-induced changes in the distribution and density of the transient outward current, 1(A), which is encoded withinthe STG by the shal gene and plays an important role in shaping rhythmic bursting of pyloric neurons. Using an antibody against lobster shal K+ channels, we found shal immunoreactivity in the membranes of neuritic processes,but not somata, of STG neurons in 5 d cultured STNS with intact modulatoryinputs. However, in 5 d decentralized STG, shal immunoreactivity was stillseen in primary neurites but was likewise present in a subset of STG somata. Among the neurons displaying this altered shal localization was the pyloric dilator (PD) neuron, which remained rhythmically active in 5 d decentralized STG. Two-electrode voltage clamp was used to compare I-A in synaptically isolated PD neurons in long-term decentralized STG and nondecentralizedcontrols. Although the voltage dependence and kinetics of I-A changed little with decentralization, the maximal conductance of I-A in PD neurons increased by 43.4%. This increase was consistent with the decentralization-induced increase in shal protein expression, indicating an alteration in the density and distribution of functional A-channels. Our results suggest that, in addition to the short-term regulation of network function, modulatory inputs may also play a role, either directly or indirectly, in controlling channel number and distribution, thereby maintaining the biophysical character of neuronal targets on a long-term basis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:37:24