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Titolo:
Endothelin-1 synthesis and endothelin B receptor expression in human coronary artery smooth muscle cells and monocyte-derived macrophages is up-regulated by low density lipoproteins
Autore:
Haug, C; Schmid-Kotsas, A; Zorn, U; Schuett, S; Gross, HJ; Gruenert, A; Bachem, MG;
Indirizzi:
Univ Hosp Ulm, Inst Clin Chem, D-89070 Ulm, Germany Univ Hosp Ulm Ulm Germany D-89070 , Inst Clin Chem, D-89070 Ulm, Germany
Titolo Testata:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
fascicolo: 9, volume: 33, anno: 2001,
pagine: 1701 - 1712
SICI:
0022-2828(200109)33:9<1701:ESAEBR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCAVENGER RECEPTOR; VASOCONSTRICTOR PEPTIDE; CHROMOSOMAL ASSIGNMENT; GROWTH-FACTORS; MESSENGER-RNA; OXIDIZED-LDL; A RECEPTOR; VITAMIN-E; SECRETION; ATHEROSCLEROSIS;
Keywords:
endothelin; LDL; coronary artery; smooth muscle cells; monocyte; macrophage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Haug, C Univ Hosp Ulm, Inst Clin Chem, Robert Koch Str 8, D-89070 Ulm, Germany Univ Hosp Ulm Robert Koch Str 8 Ulm Germany D-89070 Ulm, Germany
Citazione:
C. Haug et al., "Endothelin-1 synthesis and endothelin B receptor expression in human coronary artery smooth muscle cells and monocyte-derived macrophages is up-regulated by low density lipoproteins", J MOL CEL C, 33(9), 2001, pp. 1701-1712

Abstract

Endothelin-1 (ET-1) is a potent vasoconstrictive peptide exerting its effects predominantly by paracrine and autocrine mechanisms. ET-1 acts as a mitogen and co-mitogen on vascular smooth muscle cells, and accumulating evidence suggests that ETA is involved in the pathogenesis of atherosclerosis. Deposition of low density lipoproteins (LDL) in the vessel wall is known to play a crucial role in the development of atherosclerotic lesions. In the present study, we have investigated the effect of native LDL (nLDL) and oxidatively modified LDL (oxLDL) on ET-1 synthesis and endothelin receptor expression in cultured human coronary artery smooth muscle cells and human monocyte-derived macrophages. Native LDL and oxLDL induced a significant stimulation of ET-1 release and ET-1 mRNA expression in human coronary artery smooth muscle cells and monocyte-derived macrophages. Antibodies against the scavenger receptor CD 36 significantly reduced the oxLDL-induced stimulationof ET-1 synthesis. The antioxidants trolox and probucol did not significantly inhibit the LDL-induced rise of ET-1 release. Endothelin B receptor expression was up-regulated in both cell types after incubation with nLDL and oxLDL. In coronary smooth muscle cells, endothelin A receptor expression was slightly increased by LDL, whereas endothelin A receptor was not detectable in monocyte-derived macrophages. Coronary artery smooth muscle cells secreted a more than 150-fold higher amount of immunoreactive ET-1 into the cell culture medium than monocyte-derived macrophages. In summary, the present data. demonstrating a LDL-induced upregulation of the endothelin system in coronary smooth muscle cells and in monocyte-derived macrophages, providefurther support for a pathophysiological role of endothelin in coronary atherosclerosis and suggest that ET-1 might be involved in mediating the atherogenic effects of LDL. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:33:31