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Titolo:
Mammalian and viral IL-10 enhance C-C chemokine receptor 5 but down-regulate C-C chemokine receptor 7 expression by myeloid dendritic cells: Impact on chemotactic responses and in vivo homing ability
Autore:
Takayama, T; Morelli, AE; Onai, N; Hirao, M; Matsushima, K; Tahara, H; Thomson, AW;
Indirizzi:
Univ Pittsburgh, Med Ctr, Dept Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 pt Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh, Thomas E Starzl Transplantat Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 at Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Biochem, Pittsburgh, PA 15213 USA Univ Tokyo, Sch Med, Dept Mol Prevent Med, Tokyo, Japan Univ Tokyo TokyoJapan kyo, Sch Med, Dept Mol Prevent Med, Tokyo, Japan Univ Tokyo, Dept Surg & Bioengn, Inst Med Sci, Tokyo, Japan Univ Tokyo Tokyo Japan Dept Surg & Bioengn, Inst Med Sci, Tokyo, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 12, volume: 166, anno: 2001,
pagine: 7136 - 7143
SICI:
0022-1767(20010615)166:12<7136:MAVIEC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; MOUSE BONE-MARROW; T-CELLS; CUTTING EDGE; TOLERANCE INDUCTION; LYMPH-NODES; TNF-ALPHA; INTERLEUKIN-10; MATURE; MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Thomson, AW Univ Pittsburgh, Med Ctr, Dept Surg, W1544 Biomed Sci Tower,200 Lothrop St, Pittsburgh, PA 15213 USA Univ Pittsburgh W1544 Biomed Sci Tower,200 Lothrop St Pittsburgh PA USA 15213
Citazione:
T. Takayama et al., "Mammalian and viral IL-10 enhance C-C chemokine receptor 5 but down-regulate C-C chemokine receptor 7 expression by myeloid dendritic cells: Impact on chemotactic responses and in vivo homing ability", J IMMUNOL, 166(12), 2001, pp. 7136-7143

Abstract

The immunosuppressive and anti-inflammatory cytokine IL-10 inhibits the phenotypic and functional maturation of dendritic cells (DC) and has been reported to confer tolerogenic properties on these important professional APC. Here, we exposed murine bone marrow-derived myeloid DC to either mouse (m)or viral (v) IL-10 early during their in vitro generation in response to GM-CSF and IL-4. Both mIL-10 and vIL-10 down-regulated the expression of CCR7 mRNA determined by RT-PCR, while mIL-10 up-regulated the expression of CCR5 transcripts. These changes in CCR7 and CCR5 expression were associated with inhibition and augmentation, respectively, of DC chemotaxis toward their respective agonists, macrophage inflammatory proteins 3 beta and 1 alpha,while in vivo homing of DC from peripheral s.c. sites to secondary lymphoid tissue of syngeneic or allogeneic recipients was significantly impaired. Anti-mIL-10R mAb reversed the effects of mIL-10 on CCR expression and restored DC homing ability. Retroviral transduction of mIL-10- and vIL-10-treated DC to overexpress transgenic CCR7 partially restored the cells' lymphoid tissue homing ability in allogeneic recipients. However, CCR7 gene transferdid not reinstate the capacity of IL-10-treated DC to prime host naive T cells for ex vivo proliferative responses or Th1 cytokine (IFN-gamma) production in response to rechallenge with (donor) alloantigen. These findings suggest that in addition to their capacity to subvert DC maturation/function and confer tolerogenic potential on these cells, mIL-10 and vIL-10 regulateDC migratory responses via modulation of CCR expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:17:32