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Titolo:
Liver-derived DEC205(+)B220(+)CD19(-) dendritic cells regulate T cell responses
Autore:
Lu, LN; Bonham, CA; Liang, XY; Chen, ZY; Li, W; Wang, LF; Watkins, SC; Nalesnik, MA; Schlissel, MS; Demestris, AJ; Fung, JJ; Qian, SG;
Indirizzi:
Univ Pittsburgh, Med Ctr, Thomas E Starzl Transplantat Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 at Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh, Med Ctr, Dept Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 pt Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh, Med Ctr, Dept Cell Biol & Physiol, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Physiol, Pittsburgh, PA 15213 USA Univ Pittsburgh, Med Ctr, Dept Pathol, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Pathol, Pittsburgh, PA 15213 USA Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 12, volume: 166, anno: 2001,
pagine: 7042 - 7052
SICI:
0022-1767(20010615)166:12<7042:LDDCRT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; MULTIPOTENTIAL HEMATOPOIETIC PROGENITORS; BONE-MARROW; B-CELLS; IN-VITRO; INTERLEUKIN-3-DEPENDENT PROLIFERATION; CD40 LIGATION; MOUSE; DIFFERENTIATION; PRECURSORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, LN Univ Pittsburgh, Med Ctr, Thomas E Starzl Transplantat Inst, E1554 Biomed Sci Tower,200 Lothrop St, Pittsburgh, PA 15213 USA Univ Pittsburgh E1554 Biomed Sci Tower,200 Lothrop St Pittsburgh PA USA 15213
Citazione:
L.N. Lu et al., "Liver-derived DEC205(+)B220(+)CD19(-) dendritic cells regulate T cell responses", J IMMUNOL, 166(12), 2001, pp. 7042-7052

Abstract

Leukocytes resident in the liver may play a role in immune responses. We describe a cell population propagated from mouse liver nonparenchymal cells in IL-3 and anti-CD40 mAb that exhibits a distinct surface immunophenotype and function in directing differentiation of naive allogeneic T cells. After culture, such cells are DEC-205(bright)B220(+)CD11c(-)CD19(-), and negative for T (CD3, CD4, CD8 alpha), NK (NK 1.1) cell markers, and myeloid Ags (CD11b, CD13, CD14). These liver-derived DEC205(+)B220(+) CD19- cells have amorphology and migratory capacity similar to dendritic cells. Interestingly, they possess Ig gene rearrangements, but lack Ig molecule expression on the cell surface. They induce low thymidine uptake of allogeneic T cells inMLR due to extensive apoptosis of activated T cells. T cell proliferation is restored by addition of the common caspase inhibitor peptide, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (zVAD-fmk). T cells stimulated by liver-derived DEC205(+)B220(+)D19(-) cells release both IL-10 and IFN-gamma,small amounts of TGF-beta, and no IL-2 or IL-4, a cytokine profile resembling T regulatory type 1 cells. Expression of IL-10 and IFN-gamma, but not bioactive IL-12 in liver DEC205(+)B220(+)CD19(-) cells was demonstrated by RNase protection assay. In vivo administration of liver DEC205+B220+CD19- cells significantly prolonged the survival of vascularized cardiac allograftsin an alloantigen-specific manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 15:26:51