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Titolo:
The potency and durability of DNA- and protein-based vaccines against Leishmania major evaluated using low-dose, intradermal challenge
Autore:
Mendez, S; Gurunathan, S; Kamhawi, S; Belkaid, Y; Moga, MA; Skeiky, YAW; Campos-Neto, A; Reed, S; Seder, RA; Sacks, D;
Indirizzi:
NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA20892 Parasit Dis Lab, NIH, Bethesda, MD 20892 USA NIAID, Clin Immunol Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 Clin Immunol Lab, NIH, Bethesda, MD 20892 USA Infect Dis Res Inst, Seattle, WA 98104 USA Infect Dis Res Inst Seattle WAUSA 98104 Res Inst, Seattle, WA 98104 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 8, volume: 166, anno: 2001,
pagine: 5122 - 5128
SICI:
0022-1767(20010415)166:8<5122:TPADOD>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD8(+) T-CELLS; CUTANEOUS LEISHMANIASIS; IMMUNE-RESPONSE; PROTECTIVE IMMUNITY; PLASMID DNA; CIRCUMSPOROZOITE PROTEIN; MURINE LEISHMANIASIS; GENETIC IMMUNIZATION; CELLULAR-IMMUNITY; INTERFERON-GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Sacks, D NIAID, Parasit Dis Lab, NIH, Bldg 4,Room 126,Ctr Dr MSC 0425, Bethesda, MD20892 USA NIAID Bldg 4,Room 126,Ctr Dr MSC 0425 Bethesda MD USA 20892 2 USA
Citazione:
S. Mendez et al., "The potency and durability of DNA- and protein-based vaccines against Leishmania major evaluated using low-dose, intradermal challenge", J IMMUNOL, 166(8), 2001, pp. 5122-5128

Abstract

DNA- and protein- based vaccines against cutaneous leishmaniasis due to Leishmania major were evaluated using a challenge model that more closely reproduces the pathology and immunity associated with sand fly-transmitted infection. C57BL/6 mice were vaccinated s.c. with a mixture of plasmid DNAs encoding the Leishmania Ags LACK, LmSTI1, and TSA (AgDNA), or with autoclavedL major promastigotes (ALM) plus rIL-12, and the mice were challenged by inoculation of 100 metacyclic promastigotes in the ear dermis. When challenged at 2 wk postvaccination, mice receiving AgDNA or ALM/rIL-12 were completely protected against the development of dermal lesions, and both groups had a 100-fold reduction in peak dermal parasite loads compared with controls. When challenged at 12 wk, mice vaccinated with ALM/rIL-12 maintained partial protection against dermal lesions and their parasite loads were no longer significantly reduced, whereas the mice vaccinated with AgDNA remained completely protected and had a 1000-fold reduction in dermal parasite loads. Mice vaccinated with AgDNA also harbored few, if any, parasites in the skin during the chronic phase, and their ability to transmit L major to vectorsand flies was completely abrogated. The durable protection in mice vaccinated with AgDNA was associated with the recruitment of both CD8(+) and CD4() T cells to the site of intradermal challenge and with IFN-gamma production by CD8(+) T cells in lymph nodes draining the challenge site. These datasuggest that under conditions of natural challenge, DNA vaccination has the capacity to confer complete protection against cutaneous leishmaniasis and to prevent the establishment of infection reservoirs. The Journal of Immunology, 2001.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:44:45