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Titolo:
Unstimulated human CD4 lymphocytes express a cytoplasmic immature form of the common cytokine receptor gamma-chain
Autore:
Bani, L; Pasquier, V; Kryworuchko, M; Salamero, J; Theze, J;
Indirizzi:
Inst Pasteur, Dept Immunol, Unite Immunogenet Cellulaire, F-75724 Paris 15, France Inst Pasteur Paris France 15 ogenet Cellulaire, F-75724 Paris 15, France Inst Curie, CNRS, UMR 144, Lab Mecanismes Mol Transport Intracellulaire, F-75231 Paris, France Inst Curie Paris France F-75231 t Intracellulaire, F-75231 Paris, France
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 167, anno: 2001,
pagine: 344 - 349
SICI:
0022-1767(20010701)167:1<344:UHCLEA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
IL-2 RECEPTOR; CELLS; BETA; APOPTOSIS; SUBUNIT; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Theze, J Inst Pasteur, Dept Immunol, Unite Immunogenet Cellulaire, 25 Rue Dr Roux, F-75724 Paris 15, France Inst Pasteur 25 Rue Dr Roux Paris France15 724 Paris 15, France
Citazione:
L. Bani et al., "Unstimulated human CD4 lymphocytes express a cytoplasmic immature form of the common cytokine receptor gamma-chain", J IMMUNOL, 167(1), 2001, pp. 344-349

Abstract

As a component of various cytokine receptors, common cytokine receptor gamma -chain (gamma (c)) is essential in the development of the immune system and plays an important role in different stages of inflammatory and immune responses. Here we establish that resting CD4 T cells and the Jurkat CD4 T cell line do not express the mature form of gamma (c) (64 kDa) recognized by mAb Tugh4. However, these cells constitutively transcribe the corresponding gamma (c) gene. This apparent paradox was solved by the demonstration that polyclonal anti-gamma (c) Abs detected endoglycosidase-H-sensitive immature forms of gamma (c) (54-58 kDa) expressed by quiescent CD4 T lymphocytesand Jurkat cells. Immature gamma (c) is characterized as an intracellular component localized in the endoplasmic reticulum. Pulse-chase analysis shows that the immature gamma (c) is rapidly degraded after synthesis. After activation of CD4 T lymphocytes, and as seen in the CD4 T cell line Kit 225, the endoglycosidase-H-resistant mature form of gamma (c), is detectable at the cell surface and in the endosomal compartment. For the first time, our results demonstrate that a cytokine receptor chain may be constitutively produced as an immature form. Furthermore, this supports the notion that expression of the functional form of gamma (c), may require intracellular interactions with lineage- or subset-specific molecular partners.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 08:06:17