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Titolo:
Acetylcholine receptor peptide recognition in HLA DR3-transgenic mice: In vivo responses correlate with MHC-peptide binding
Autore:
Raju, R; Spack, EG; David, CS;
Indirizzi:
St Lukes Hosp, Div Immunol, Milwaukee, WI 53215 USA St Lukes Hosp Milwaukee WI USA 53215 Div Immunol, Milwaukee, WI 53215 USA Corixia Corp, Dept Immunol, Redwood City, CA 94010 USA Corixia Corp Redwood City CA USA 94010 mmunol, Redwood City, CA 94010 USA Mayo Clin & Mayo Grad Sch Med, Dept Immunol, Rochester, MN 55905 USA Mayo Clin & Mayo Grad Sch Med Rochester MN USA 55905 hester, MN 55905 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 167, anno: 2001,
pagine: 1118 - 1124
SICI:
0022-1767(20010715)167:2<1118:ARPRIH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYASTHENIA-GRAVIS; T-CELLS; TRANSGENIC MICE; ALPHA-SUBUNIT; MOLECULES; EPITOPE; HETEROGENEITY; ANTIGEN; CD4(+); SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Raju, R St Lukes Hosp, Div Immunol, 2900 W Oklahoma Ave, Milwaukee, WI 53215 USA St Lukes Hosp 2900 W Oklahoma Ave Milwaukee WI USA 53215 53215 USA
Citazione:
R. Raju et al., "Acetylcholine receptor peptide recognition in HLA DR3-transgenic mice: In vivo responses correlate with MHC-peptide binding", J IMMUNOL, 167(2), 2001, pp. 1118-1124

Abstract

HLA DR3 is an MHC molecule that reportedly predisposes humans to myasthenia gravis (MG). Though MG is an Ab-mediated autoimmune disease, CD4(+) T cells are essential for the generation of high-affinity Abs; hence the specificities of autoreactive CD4(+) T cells are important. In this study we report the HLA DR3-restricted T cell determinants on the extracellular region sequence of human acetylcholine receptor a subunit. We find two promiscuous determinants on this region 141-160 and 171-190 as defined by their immunogenicity in HLA DR3-, HLA DQ8-, and HLA DQ6-transgenic mice in the absence ofendogenous mouse class II molecules. We also studied the minimal determinants of these two regions by truncation analysis, and the MHC binding affinity of a set of overlapping peptides spanning the complete sequence region of human acetylcholine receptor a subunit. One of the peptide sequences strongly immunogenic in HLA DR3-transgenic mice also had the highest binding affinity to HLA DR3. Identification of T cell determinants restricted to an MHC molecule known to predispose to MG may be an important step toward the development of peptide-based immunomodulation strategies for this autoimmunedisease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:39:48