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Titolo:
Sensitized mast cells migrate toward the agen: A response regulated by p38mitogen-activated protein kinase and Rho-associated coiled-coil-forming protein kinase
Autore:
Ishizuka, T; Okajima, F; Ishiwara, M; Iizuka, K; Ichimonji, I; Kawata, T; Tsukagoshi, H; Dobashi, K; Nakazawa, T; Mori, M;
Indirizzi:
Gunma Univ, Sch Med, Dept Internal Med 1, Maebashi, Gumma 3718511, Japan Gunma Univ Maebashi Gumma Japan 3718511 1, Maebashi, Gumma 3718511, Japan Gunma Univ, Inst Mol & Cellular Regulat, Lab Signal Transduct, Maebashi, Gumma, Japan Gunma Univ Maebashi Gumma Japan Signal Transduct, Maebashi, Gumma, Japan Gunma Univ, Inst Mol & Cellular Regulat, Fac Hlth Sci, Maebashi, Gumma, Japan Gunma Univ Maebashi Gumma Japan at, Fac Hlth Sci, Maebashi, Gumma, Japan Dainippon Ink & Chem, Cent Res Labs, Sakura, Japan Dainippon Ink & Chem Sakura Japan & Chem, Cent Res Labs, Sakura, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 4, volume: 167, anno: 2001,
pagine: 2298 - 2304
SICI:
0022-1767(20010815)167:4<2298:SMCMTT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; HUMAN ENDOTHELIAL-CELLS; HEAT-SHOCK PROTEINS; FC-EPSILON-RI; MAPKAP KINASE-2; STIMULATES PROLIFERATION; ALPHA PRODUCTION; PHOSPHORYLATION; INHIBITOR; REORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ishizuka, T Gunma Univ, Sch Med, Dept Internal Med 1, 3-39-15 Showa Machi,Maebashi, Gumma 3718511, Japan Gunma Univ 3-39-15 Showa Machi Maebashi Gumma Japan 3718511 an
Citazione:
T. Ishizuka et al., "Sensitized mast cells migrate toward the agen: A response regulated by p38mitogen-activated protein kinase and Rho-associated coiled-coil-forming protein kinase", J IMMUNOL, 167(4), 2001, pp. 2298-2304

Abstract

Although mast cells accumulate within the mucosal epithelial layer of patients with allergic rhinitis and bronchial asthma, the responsible chemotactic factors are undefined. We investigated whether mast cells sensitized with Ag-specific IgE migrate toward the Ag. MC/9 mast cells sensitized with anti-DNP IgE migrated toward DNP-conjugated human serum albumin. This migration was directional, and the degree was stronger than that induced by stem cell factor. IL-3 and stem cell factor-dependent cultured mast cells derivedfrom mouse bone marrow also migrated toward the Ag. Subsequent migration mediated by the Fc epsilon RI was significantly inhibited by incubating the cells with Y-27632, a Rho-associated coiled-coil-forming protein kinase inhibitor, or with SB203580, a p38 mitogen-activated protein kinase (MAPK) inhibitor. Both p38 MAPK and MAPK-activated protein kinase (MAPKAPK)2 were activated following Fc epsilon RI aggregation, and activation of MAPKAPK2 was almost completely inhibited by 10 muM SB203580. Wortmannin or a low concentration of SB203580 partially inhibited MAPKAPK2, but did not block mast cell migration. In contrast, Y-27632 did not affect the activation of MAPKAPK2. These results indicate that Ag works not only as a stimulant for allergicmediators from IgE-sensitized mast cells, but also as a chemotactic factorfor mast cells. Both p38 MAPK activation and Rho-dependent activation of Rho-associated coiled-coil-forming protein kinase may be required for Fc epsilon RI-mediated cell migration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:31:41