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Titolo:
Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecularcloning and characterization of trehalose-6-phosphate synthase homologues
Autore:
Vogel, G; Fiehn, O; Jean-Richard-dit-Bressel, L; Boller, T; Wiemken, A; Aeschbacher, RA; Wingler, A;
Indirizzi:
Univ Basel, Inst Bot, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 nst Bot, CH-4056 Basel, Switzerland Max Planck Inst Mol Plant Physiol, D-14424 Potsdam, Germany Max Planck Inst Mol Plant Physiol Potsdam Germany D-14424 tsdam, Germany
Titolo Testata:
JOURNAL OF EXPERIMENTAL BOTANY
fascicolo: 362, volume: 52, anno: 2001,
pagine: 1817 - 1826
SICI:
0022-0957(200109)52:362<1817:TMIAOO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC TOBACCO PLANTS; SACCHAROMYCES-CEREVISIAE; SELAGINELLA-LEPIDOPHYLLA; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; YEAST; GENE; LEAVES; STRESS; IDENTIFICATION;
Keywords:
Arabidopsis; trehalose; trehalose-6-phosphate phosphatase; trehalose-6-phosphate synthase; yeast complementation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Vogel, G Kantonales Lab Basel Stadt Kontrollstelle Chem &, Missionsstr 60,CH-4012 Basel, Switzerland Kantonales Lab Basel Stadt Kontrollstelle Chem & Missionsstr 60 Basel Switzerland CH-4012
Citazione:
G. Vogel et al., "Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecularcloning and characterization of trehalose-6-phosphate synthase homologues", J EXP BOT, 52(362), 2001, pp. 1817-1826

Abstract

Axenically grown Arabidopsis thaliana plants were analysed for the occurrence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from Arabidopsis inflorescences. In a variety of organisms, the synthesis of trehalose is catalysed by trehalose-6-phosphate synthase (TPS; EC 2.4.1.15) and trehalose-6-phosphate phosphatase (TPP; EC 3.1.3.12). Based on EST (expressed sequence tag) sequences, three full-length Arabidopsis cDNAs whose predicted protein sequences show extensive homologies to known TPS and TPP proteins were amplified by RACE-PCR. The expression of the corresponding genes, AtTPSA, AtTPSB and AtTPSC, and of the previously described TPS gene, AtTPS1, was analysed by quantitative RT-PCR. All of the genes were expressed in the rosette leaves, stems and flowers of Arabidopsis plants and, to a lower extent, in the roots. To study the role of the Arabidopsis genes, the AtTPSA and AtTPSC cDNAs were expressed in Saccharomyces cerevisiae mutants deficient in trehalose synthesis. In contrast to AtTPS1, expression of AtTPSA and AtTPSC in the tps1 mutant lacking TPS activity did not complement trehalose formation after heat shock or growth on glucose. In addition, no TPP function could be identified for AtTPSA and AtTPSC in complementation studies with the S. cerevisiae tps2 mutant lacking TPP activity. The results indicate that whileAtTPS1 is involved in the formation of trehalose in Arabidopsis, some of the Arabidopsis genes with homologies to known TPS/TPP genes encode proteinslacking catalytic activity in trehalose synthesis.

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Documento generato il 08/04/20 alle ore 09:09:26