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Titolo:
Development of a diagnostic DNA probe for the fruit flies Ceratitis capitata and Ceratitis rosa (Diptera : Tephritidae) using amplified fragment-length polymorphism
Autore:
Kakouli-Duarte, T; Casey, DG; Burnell, AM;
Indirizzi:
Natl Univ Ireland Maynooth, Inst Bioengn & Agroecol, Dept Biol, Maynooth, Kildare, Ireland Natl Univ Ireland Maynooth Maynooth Kildare Ireland th, Kildare, Ireland
Titolo Testata:
JOURNAL OF ECONOMIC ENTOMOLOGY
fascicolo: 4, volume: 94, anno: 2001,
pagine: 989 - 997
SICI:
0022-0493(200108)94:4<989:DOADDP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFLP MARKERS; RIBOSOMAL DNA; NEMATODE RESISTANCE; GENETIC DIVERSITY; PARTIAL SEQUENCES; FLY DIPTERA; IDENTIFICATION; POTATO; TOOL; BIODIVERSITY;
Keywords:
Ceratitis capitata; Ceratitis rosa; amplified fragment-length polymorphism; silver staining; DNA probes; tephritids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kakouli-Duarte, T Natl Univ Ireland Maynooth, Inst Bioengn & Agroecol, Dept Biol, Maynooth, Kildare, Ireland Natl Univ Ireland Maynooth Maynooth Kildare Ireland nd
Citazione:
T. Kakouli-Duarte et al., "Development of a diagnostic DNA probe for the fruit flies Ceratitis capitata and Ceratitis rosa (Diptera : Tephritidae) using amplified fragment-length polymorphism", J ECON ENT, 94(4), 2001, pp. 989-997

Abstract

The AFLP technique (amplified fragment-length polymorphism) was employed to identify and isolate species specific markers in tephritids. We have found that the technique has good potential for this purpose, with the only difficult part being the reamplification of AFLP fragments from silver stainedgels. Cloning of putative species-specific markers and genomic dot blot hybridizations resulted in the development of diagnostic probes for tephritididentification. A repetitive DNA sequence from the genome of Ceratitis capitata (Wiedemann) was isolated. This sequence rapidly and reliably identified C. capitata and C. rosa Karsch in a collection of closely related and outgroup species tested in this study. Although this probe has been developedfor C. capitata and C. rosa, the proposed methodology can be applied to any group of organisms.

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Documento generato il 18/09/20 alle ore 10:57:11