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Titolo:
A new and versatile method for determination of thiolamines of biological importance
Autore:
Dominick, PK; Cassidy, PB; Roberts, JC;
Indirizzi:
Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Chem, Salt Lake City, UT 84112 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 761, anno: 2001,
pagine: 1 - 12
SICI:
1387-2273(20010915)761:1<1:ANAVMF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; RAT HEPATOCYTES; CELL-LINE; GLUTATHIONE; DISULFIDES; PRODRUGS; THIOLS;
Keywords:
thiolamines; glutathione; cystine; cysteine; glutathione disulfide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, JC Univ Utah, Dept Med Chem, 30 S 2000 East,Room 201, Salt Lake City, UT 84112 USA Univ Utah 30 S 2000 East,Room 201 Salt Lake City UT USA 84112
Citazione:
P.K. Dominick et al., "A new and versatile method for determination of thiolamines of biological importance", J CHROMAT B, 761(1), 2001, pp. 1-12

Abstract

A method for the separation and quantitation of several important biological thiolamines is described. The procedure employs a C-18 reversed-phase HPLC system to separate the dinitrophenyl derivatives of reduced and oxidizedglutathione and cysteine and relies on an internal standard, N-epsilon-methyllysine, to minimize experimental error. The method was validated in three matrices (water, HepG2 cell lysates, and mouse liver homogenates) using several criteria. The detector response was linear for the dinitrophenyl derivatives of glutathione, glutathione disulfide, cysteine, and cystine in the concentrations ranging from 10 to 50 nmol/ml. Inter- and intra-day variation, percent recovery in the biological matrices, and limits of detection and quantitation were determined. For the most accurate determination, it isessential that standard curves be produced daily and in the same matrix asthat being analyzed. (C) 2001 Elsevier Science BM All rights reserved.

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Documento generato il 30/03/20 alle ore 18:40:47