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Titolo:
Pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of alpha-ketoisocaproate for isoamyl alcohol formation in Saccharomyces cerevisiae
Autore:
Yoshimoto, H; Fukushige, T; Yonezawa, T; Sakai, Y; Okawa, K; Iwamatsu, A; Sone, H; Tamai, Y;
Indirizzi:
Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Kanagawa Japan 2360004 Ku, Kanagawa 2360004, Japan Kirin Seagram Ltd, Gotemba Plant, Shizuoka 4120003, Japan Kirin Seagram Ltd Shizuoka Japan 4120003 Plant, Shizuoka 4120003, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 1, volume: 92, anno: 2001,
pagine: 83 - 85
SICI:
1389-1723(200107)92:1<83:PDEBTP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST; EXPRESSION;
Keywords:
decarboxylase; alpha-ketoisocaproate; isoamyl alcohol; pyruvate decarboxylase; PDC1 gene; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshimoto, H Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, 1-13-5 Fukuura, Kanagawa 2360004, Japan Kirin Brewery Co Ltd 1-13-5 Fukuura Kanagawa Japan 2360004 n
Citazione:
H. Yoshimoto et al., "Pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of alpha-ketoisocaproate for isoamyl alcohol formation in Saccharomyces cerevisiae", J BIOSCI BI, 92(1), 2001, pp. 83-85

Abstract

Isoamyl alcohol is an important flavor component of yeast-fermented alcoholic beverages. To identify the enzyme and gene involved in the decarboxylation of alpha -ketoisocaproate (alpha -KIC) for isoamyl alcohol formation, the enzyme was partially purified and analyzed by mass spectrometry. The pyruvate decarboxylase encoded by the PDC1 gene was considered a likely candidate enzyme. Genetic analysis showed that the activity of alpha -KIC decarboxylase and production of isoamyl alcohol partially decreased in a pdc1 nullmutant and increased in a transformant with a multi-copy plasmid carrying the PDC1 gene. These results indicate that pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of alpha -KIC for isoamyl alcohol formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:53:58