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Titolo:
Chromatin remodeling by the thyroid hormone receptor in regulation of the thyroid-stimulating hormone alpha-subunit promoter
Autore:
Collingwood, TN; Urnov, FD; Chatterjee, VKK; Wolffe, AP;
Indirizzi:
NIH, Mol Embryol Lab, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892NIH, Mol Embryol Lab, Bethesda, MD 20892 USA Univ Cambridge, Addenbrookes Hosp, Dept Med, Cambridge CB2 2QQ, England Univ Cambridge Cambridge England CB2 2QQ Med, Cambridge CB2 2QQ, England
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 36, volume: 276, anno: 2001,
pagine: 34227 - 34234
SICI:
0021-9258(20010907)276:36<34227:CRBTTH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SPECIFIC EXPRESSION; TUMOR VIRUS PROMOTER; BETA-A GENE; NUCLEAR RECEPTOR; POSITIONED NUCLEOSOMES; TRANSCRIPTION FACTOR; IN-VIVO; HYPERSENSITIVE SITES; BASAL TRANSCRIPTION; NEGATIVE REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Collingwood, TN Sangamo Biosci, 501 Canal Blvd,Suite A100, Richmond, CA 94804 USA Sangamo Biosci 501 Canal Blvd,Suite A100 Richmond CA USA 94804
Citazione:
T.N. Collingwood et al., "Chromatin remodeling by the thyroid hormone receptor in regulation of the thyroid-stimulating hormone alpha-subunit promoter", J BIOL CHEM, 276(36), 2001, pp. 34227-34234

Abstract

The chromatin architecture of a promoter is an important determinant of its transcriptional response. For most target genes, the thyroid hormone receptor (TR) activates gene expression in response to thyroid hormone (T-3). In contrast, the thyroid-stimulating hormone a-subunit (TSH alpha) gene promoter is down-regulated by TR in the presence of T-3. Here we utilize the capacity for the Xenopus oocyte to chromatinize exogenous nuclear-injected DNA to analyze the chromatin architecture of the TSHa promoter and how this changes upon TR-mediated regulation. Interestingly, in the oocyte, the TSH alpha promoter was positively regulated by T-3. In the inactive state, the promoter contained six loosely positioned nucleosomes. The addition of TR/retinoid X receptor together had no effect on the chromatin structure, but the inclusion of T-3 induced strong positioning of a dinucleosome in the TSHaproximal promoter that was bordered by regions that were hypersensitive tocleavage by methidiumpropyl EDTA. We identified a novel thyroid response element that coincided with the proximal hypersensitive region. Furthermore,we examined the consequences of mutations in TR that impaired coactivator recruitment. In a comparison with the Xenopus TR betaA promoter, we found that the effects of these mutations on transactivation and chromatin remodeling were significantly more severe on the TSH alpha promoter.

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Documento generato il 30/03/20 alle ore 07:45:36