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Titolo:
Molecular cloning and characterization of human nonsteroidal anti-inflammatory drug-activated gene promoter - Basal transcription is mediated by Sp1 and Sp3
Autore:
Baek, SJ; Horowitz, JM; Eling, TE;
Indirizzi:
NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA N Carolina State Univ, Coll Vet Med, Dept Anat Phys Sci & Radiol, Raleigh,NC 27606 USA N Carolina State Univ Raleigh NC USA 27606 & Radiol, Raleigh,NC 27606 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 36, volume: 276, anno: 2001,
pagine: 33384 - 33392
SICI:
0021-9258(20010907)276:36<33384:MCACOH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA SUPERFAMILY; GROWTH-FACTOR-BETA; FUNCTIONAL INTERACTIONS; REPRESS TRANSCRIPTION; MORPHOGENETIC PROTEIN; BINDING PROTEINS; CARCINOMA-CELLS; RB PROTEIN; SP-FAMILY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Eling, TE NIEHS, Mol Carcinogenesis Lab, NIH, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA NIEHS 111 TW Alexander Dr Res Triangle Pk NC USA 27709 27709 USA
Citazione:
S.J. Baek et al., "Molecular cloning and characterization of human nonsteroidal anti-inflammatory drug-activated gene promoter - Basal transcription is mediated by Sp1 and Sp3", J BIOL CHEM, 276(36), 2001, pp. 33384-33392

Abstract

Nonsteroidal anti-inflammatory drug-activated gene (NAG-1) is known to be associated with anti-tumorigenic activity and belongs to the transforming growth factor-beta superfamily. In the present study, we cloned the promoterregion (-3500 to +41) and investigated the transcriptional regulatory mechanisms of the basal expression of the human NAG-1 gene. Several potential transcription factor-binding sites in this region were identified. Based on the results from clones of nested deletions, the construct between -133 and+41 base pairs contains three Sp1-binding sites (Sp1-A, Sp1-B, and Sp1-C, which confer basal transcription specific activity of NAG-1 expression. When the Spi-C site was mutated (GG to TT), a 60-80% decrease in promoter activity was observed in HCT-116 cells. Gel shift, cotransfection, and chromatin immunoprecipitation assays showed that the Sp transcription factors bind to the Spl-binding sites and transactivate NAG-1 expression. In addition, chicken ovalbumin upstream promoter transcription factor I can interact withthe C-terminal region of Sp1 and Sp3 proteins and induce NAG-1 promoter activity through SpI and Sp3 transcription factors. These results identify the critical regulatory regions for the human NAG-1 basal promoter. Furthermore, the results suggest that the level of expression of the NAG-1 gene willdepend on the availability of Sp proteins and on co-factors such as chicken ovalbumin upstream promoter-transcription factor 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 04:53:38