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Titolo:
The XRCC2 human repair gene influences recombinational rearrangements leading to chromatid breaks
Autore:
Mozdarani, H; Liu, N; Jones, NJ; Bryant, PE;
Indirizzi:
Univ St Andrews, Sch Biol, St Andrews KY16 9TS, Fife, Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS Y16 9TS, Fife, Scotland Tarbiat Modarres Univ, Sch Med Sci, Tehran, Iran Tarbiat Modarres Univ Tehran Iran arres Univ, Sch Med Sci, Tehran, Iran Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA Lawrence Livermore Natl Lab Livermore CA USA 94551 ivermore, CA 94551 USA Univ Liverpool, Donnan Labs, Sch Biol Sci, Liverpool L69 7ZD, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 7ZD , Merseyside, England
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 8, volume: 77, anno: 2001,
pagine: 859 - 865
SICI:
0955-3002(200108)77:8<859:TXHRGI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND BREAKS; ATAXIA-TELANGIECTASIA CELLS; SENSITIVE IRS MUTANTS; HUMAN RAD51 PROTEIN; IONIZING-RADIATION; RESTRICTION ENDONUCLEASES; CHROMOSOMAL-ABERRATIONS; DNA-SYNTHESIS; V79 CELLS; HAMSTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Bryant, PE Univ St Andrews, Sch Biol, St Andrews KY16 9TS, Fife, Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS fe, Scotland
Citazione:
H. Mozdarani et al., "The XRCC2 human repair gene influences recombinational rearrangements leading to chromatid breaks", INT J RAD B, 77(8), 2001, pp. 859-865

Abstract

Purpose: To test the possible involvement of the XRCC2 gene in the controlof intra-versus interchromatid rearrangements leading to chromatid breaks in G(2) cells by studying the Colour-switch ratio (CSR) in harlequin-stained Chinese hamster irs1 cells. Materials and methods: The V79-1 mutant cell lines irs1 (XRCC2 mutation) and irs2 (XRCC8 mutation), two WT V79 lines and GT621-1 (irs1 transfected with the XPLC2 gene) were labelled with BrdU through two cell cycles, irradiated and sampled 1.5 h after exposure. Metaphase spreads were analysed for chromatid break frequency and frequencies of colour-switch (colour-switch between chromatids at the break point) and non-colour-switch breaks, from Which the CSR was calculated. Results: Chromatid breaks were induced linearly with dose in all lines, and frequencies were elevated in irs1 and irs2 mutant Cell lines when Compared with WT lines. An XRCC2 transfected line (GT621-1) showed full radiosensitivity complementation with respect to frequencies, of chromatid breaks. The CSR was significantly higher in irs1 (13.9%) than in the parental V79-4 (7.5%) or irs2 (4.9%) cells. GT621-1 cells showed partial, but significant complementation with respect to CSR (9.2%). Conclusions: It is concluded that the significantly higher CSR for the irs1 mutant than for the Wild-type parental V79-4 line indicates the involvement of the XRCC2 gene product in the control of the rearrangement process leading to chromatid breaks.

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Documento generato il 02/04/20 alle ore 02:06:27