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Titolo:
Time-dose relationships in radiation-enhanced integration
Autore:
Stevens, CW; Puppi, M; Cerniglia, GJ;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Radiat Oncol, Houston, TX 77030 USAUniv Texas Houston TX USA 77030 Dept Radiat Oncol, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Expt Radiat Oncol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 Expt Radiat Oncol, Houston, TX 77030 USA Univ Penn, Dept Radiat Oncol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 iat Oncol, Philadelphia, PA 19104 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 8, volume: 77, anno: 2001,
pagine: 841 - 846
SICI:
0955-3002(200108)77:8<841:TRIRI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSFER EFFICIENCY; IONIZING-RADIATION; ALPHA-PARTICLES; MAMMALIAN-CELLS; DNA-DAMAGE; THERAPY; EXPOSURE; COMPLEXITY; EXPRESSION; KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Stevens, CW Univ Texas, MD Anderson Canc Ctr, Dept Radiat Oncol, 1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd Houston TX USA 77030 X 77030 USA
Citazione:
C.W. Stevens et al., "Time-dose relationships in radiation-enhanced integration", INT J RAD B, 77(8), 2001, pp. 841-846

Abstract

Purpose: We have Shown that, ionizing radiation increases recombination, as manifested by increased Stable transduction of both plasmid and adenoviral vectors. This paper reports the duration of increased recombination afterirradiation. Materials and methods: A549 or NIH/3T3 cells were transfected at various times after irradiation. Cells were also irradiated with several fractionation schemes and then transfected. Results: Enhanced integration (El) is a very long-lived process, lasting at least 2-3 days after single radiation fractions. The duration of EI activation is radiation dose-dependent. The efficiency of EI is dependent on radiation close and independent of fractionation, such that low close-rate, fractionated and single radiation doses result in similar levels of EI when corrected for differences in cytotoxicity. Conclusions: Radiation, given with fraction sizes and dose-rates used in clinical radiation therapy, induces a Ion-lived hyper-recombination state, Since radiotherapy is already a component of treatment for many malignanciesand is integrated into radiation-gene therapy trials, an understanding of recombination events that. improve gene delivery is important and timely.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 23:48:54