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Titolo:
Production of bioengineered cancer tissue constructs in vitro: Epithelium-mesenchyme heterotypic interactions
Autore:
Wang, CS; Goulet, F; Auger, F; Tremblay, N; Germain, L; Tetu, B;
Indirizzi:
CHUQ, Hotel Dieu, Dept Pathol, Ctr Rech Cancerol, Quebec City, PQ G1R 2J6,Canada CHUQ Quebec City PQ Canada G1R 2J6 ncerol, Quebec City, PQ G1R 2J6,Canada Univ Laval, Fac Med, Ctr Hosp Affilie, Lab Organogenese Expt, Quebec City,PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 , Quebec City,PQ G1K 7P4, Canada
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 7, volume: 37, anno: 2001,
pagine: 434 - 439
SICI:
1071-2690(200107/08)37:7<434:POBCTC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS IN-VITRO; HUMAN FIBROBLASTS; BREAST-CARCINOMA; IV COLLAGENASE; TUMOR INVASION; HUMAN SKIN; EXPRESSION; MATRIX; PROLIFERATION; STROMELYSIN-3;
Keywords:
tumor-derived fibroblasts; cellular interactions; collagen matrix; breast cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Tetu, B CHUQ, Hotel Dieu, Dept Pathol, Ctr Rech Cancerol, 11 Cote Palais, Quebec City, PQ G1R 2J6, Canada CHUQ 11 Cote Palais Quebec City PQ Canada G1R 2J6 G1R 2J6, Canada
Citazione:
C.S. Wang et al., "Production of bioengineered cancer tissue constructs in vitro: Epithelium-mesenchyme heterotypic interactions", IN VITRO-AN, 37(7), 2001, pp. 434-439

Abstract

A few models have been established to study cancer cells in vitro. However. the cellular interactions have rarely been studied specifically using bioengineered cancer constructs combining human carcinoma cells and tumor-associated fibroblasts. We developed an in vitro model of tridimensional bioengineered cancer tissue constructs (bCTC) by seeding mammary epithelial cancer cells or normal keratinocytes over a mesenchymal layer containing tumor-derived fibroblastic cells or normal skin fibroblasts. After the introduction of epithelial cells, each construct was cultured for another 10 d. Histologic analyses showed that carcinoma cell lines could invade the subjacent mesenchymal layer and that the capacity to migrate was related to the invasive potential of cancer cells and the type of fibroblasts used, while noninvasive populations did not. Of the tested epithelial cells, MDA-MB-231 and, to a lesser degrees HDQ-P1 cell lines were invasive, and the invasion was deeper into the mesenchymal component containing tumor-derived fibroblasts. However, with normal skin fibroblast.,, the mesenchymal layer was degraded twice faster than with tumor-derived fibroblastic cells. MDA-MB-231 cells and normal keratinocytes induced the highest level of gelatinase B. and the level was lowest with the MCF-7 cell line. The activated form of gelatinaseB was, however, induced to the highest levels in the keratinocyte-seeded bCTC containing tumor-derived but not normal fibroblasts. MDA-MB-231 was theonly epithelial cancer cell line whose activity of gelatinase A was reduced when cocultured with tumor-derived fibroblasts but not under normal fibroblast stimulation. Finally, a 50/48-kDa gelatinase band has been observed in bCTCs with noninvasive epithelial cells only. Our study demonstrates the selective secretion of gelatinases according to the phenotype of the cells seeded in the various bCTCs.

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Documento generato il 28/09/20 alle ore 13:35:18