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Titolo:
Nonviral gene transfer into primary cultures of human and porcine mesothelial cells
Autore:
Ohan, J; Gilbert, MA; Leseche, G; Panis, Y; Midoux, P; Drouet, L;
Indirizzi:
Lab Chirurg Vasc & Thorac, Paris, France Lab Chirurg Vasc & Thorac ParisFrance urg Vasc & Thorac, Paris, France INSERM 353, Paris, France INSERM 353 Paris FranceINSERM 353, Paris, France INSERM 450, Paris, France INSERM 450 Paris FranceINSERM 450, Paris, France Hop Lariboisiere, Serv Hematol, F-75475 Paris, France Hop Lariboisiere Paris France F-75475 erv Hematol, F-75475 Paris, France Hop Lariboisiere, Serv Chirurg Gen, F-75475 Paris, France Hop Lariboisiere Paris France F-75475 Chirurg Gen, F-75475 Paris, France Ctr Biophys Mol, F-45071 Orleans, France Ctr Biophys Mol Orleans France F-45071 phys Mol, F-45071 Orleans, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 7, volume: 37, anno: 2001,
pagine: 402 - 407
SICI:
1071-2690(200107/08)37:7<402:NGTIPC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL-CELLS; FIBRINOLYTIC PROPERTIES; RECOMBINANT PROTEIN; ORGAN-CULTURE; DACRON GRAFTS; THERAPY; TISSUE; EXPRESSION; KERATINOCYTES; ASSAY;
Keywords:
gene therapy; gene transfer; nonviral vector; green fluorescent protein; luciferase; mesothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Ohan, J Hop Beaujon, Lab Chirurg Vasc & Thorac, 100 Blvd Gen Leclerc, F-92100 Boulogne, France Hop Beaujon 100 Blvd Gen Leclerc Boulogne France F-92100 , France
Citazione:
J. Ohan et al., "Nonviral gene transfer into primary cultures of human and porcine mesothelial cells", IN VITRO-AN, 37(7), 2001, pp. 402-407

Abstract

Due to their abundance and accessibility, mesothelial cells may be suitable toots for recombinant reagent expression by gene transfer. Genetically modified porcine mesothelial cells (PMCs) may have the potential for the treatment of vascular diseases in humans. We studied the effect of various transfection reagents on the primary culture of PMCs and human mesothelial cells (HMCs). The cells were transfected with a plasmid encoding a reporter gene (luciferase or green fluorescent protein [GFP]) under the control of the cytomegalovirus promoter. Transfection was achieved using cationic lipids (DOSPER and DOTAP) or calcium phosphate/deoxyribonucleic acid coprecipitation or Fugene 6. Results showed that Fugene 6 was the most efficient and reproducible transfection reagent with both PMCs and HMCs. With Fugene 6, luciferase activity in PMCs (1.5 X 10(8) relative light units [RLU]/10(6) cells)was at least 2.5-fold higher than with the other transfection reagents, and it was 100-fold higher than in HMCs. However, the proportion of transfected cells expressing GFP was only 1%. These preliminary findings open up newavenues for developing experimental studies on the use of genetically modified PMCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:56:33