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Titolo:
Nicotine affects the signaling of the death pathway, reducing the responseof head and neck cancer cell lines to DNA damaging agents
Autore:
Onoda, N; Nehmi, A; Weiner, D; Mujumdar, S; Christen, R; Los, G;
Indirizzi:
Univ Calif San Diego, Canc Ctr 0058, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 r 0058, La Jolla, CA 92093 USA Osaka City Univ, Sch Med, Dept Oncol, Inst Geriat & Med Sci,Abeno Ku, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 d Sci,Abeno Ku, Osaka 5458585, Japan
Titolo Testata:
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
fascicolo: 10, volume: 23, anno: 2001,
pagine: 860 - 870
SICI:
1043-3074(200110)23:10<860:NATSOT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA LEVEL; JUN ACTIVATION DOMAIN; FACTOR C-JUN; PROTEIN-KINASE; CIGARETTE-SMOKING; UP-REGULATION; APOPTOSIS; INDUCTION; GADD153; STRESS;
Keywords:
nicotine; cellular injury response; apoptosis; JNK; apoptosis; cytotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Los, G Univ Calif San Diego, Canc Ctr 0058, 9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 92093 USA
Citazione:
N. Onoda et al., "Nicotine affects the signaling of the death pathway, reducing the responseof head and neck cancer cell lines to DNA damaging agents", HEAD NECK, 23(10), 2001, pp. 860-870

Abstract

Background. Growing evidence suggests that tobacco can affect the responsiveness of cancer cells to treatment , particularly those of head and neck cancer. This article describes the effects of nicotine on the signaling of the death pathway, resulting in a decreased cytotoxicity of various anticancer agents such as cisplatin and gamma-radiation. Methods. Colony-forming assays (CFA), using the head and neck cancer cell lines UMSCC10b and UMSCC5 and DNA fragmentation assays, were used to determine the effect of nicotine on cytotoxicity of various anticancer agents, whereas PCR and a JNK activity test were used to study the effect of nicotineon message expression levels and activity of the JNK signaling pathway. Results. Nicotine consistently reduced the cytotoxic effect of DNA-damaging agents, such as cisplatin, UV, and gamma radiation, in UMSCC10b cells, increasing their IC50 values by twofold, 1.7-fold, and 1.8-fold, respectively. These results were confirmed in a second squamous cell carcinoma cell line (UMSCC5), demonstrating an increase in IC50 values for cDDP by twofold and 1.9-fold in the UMSCC10b and UMSCC5, respectively. In addition, nicotine reduced the DNA fragmentation 48 h after cDDP exposure in UMSCC10b and UMSCC5 cell lines by 30% and 33%, respectively. The latter, however, was not the result of an effect of nicotine on either the uptake of cDDP or repair ofthe cDDP-DNA-adducts. To further substantiate the adverse effect of nicotine, the JNK and gadd153 signaling pathways were studied. JNK activity was decreased by 1.8-fold, as well as the expression of its downstream target c-jun (1.9-fold), when tumor cells were exposed to cisplatin in the presence of nicotine. In addition, the gadd153 message was affected and reduced by 1.8-fold. Conclusions. Nicotine adversely affects the cytotoxicity of DNA-damaging agents. Nicotine does not interfere with the repair of the damage but directly affects the signaling of the death pathway, reducing the signaling of the JNK1 pathway. The latter results in a decrease in efficacy of the anticancer treatment in tumors exposed to nicotine. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:23:09