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Titolo:
Continuous interleukin-6 application in vivo via macroencapsulation of interleukin-6-expressing COS-7 cells induces massive gliosis
Autore:
Tilgner, J; Volk, B; Kaltschmidt, C;
Indirizzi:
Univ Freiburg, Dept Neuropathol, Freiburg, Germany Univ Freiburg Freiburg Germany urg, Dept Neuropathol, Freiburg, Germany Univ Witten Herdecke, Inst Neurobiochem, Witten, Germany Univ Witten Herdecke Witten Germany Inst Neurobiochem, Witten, Germany
Titolo Testata:
GLIA
fascicolo: 3, volume: 35, anno: 2001,
pagine: 234 - 245
SICI:
0894-1491(200109)35:3<234:CIAIVV>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; TUMOR-NECROSIS-FACTOR; ALZHEIMERS-DISEASE PATIENTS; RECEPTOR MESSENGER-RNA; GROWTH-FACTOR; RAT-BRAIN; CEREBROSPINAL-FLUID; TRANSGENIC MICE; NEUROGLIAL ACTIVATION; SYMPATHETIC NEURONS;
Keywords:
astrocytes; brain; encapsulation; implantation; microglia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Tilgner, J Univ Wurzburg, Dept Neurosurg, Josef Schneider Str 11, D-97080 Wurzburg, Germany Univ Wurzburg Josef Schneider Str 11 Wurzburg Germany D-97080
Citazione:
J. Tilgner et al., "Continuous interleukin-6 application in vivo via macroencapsulation of interleukin-6-expressing COS-7 cells induces massive gliosis", GLIA, 35(3), 2001, pp. 234-245

Abstract

The inflammatory cytokine interleukin-6 (IL-6) was found in senile plaquesof Alzheimer's patients and might be involved in the pathology of Parkinson's disease and multiple sclerosis. Interestingly, an astocytosis is also found in these neurodegenerative disorders. To evaluate the direct effects of IL-6 in vivo on glial cells, we created a new in vivo model. IL-6 and mock-transfected (control group) COS-7 cells were encapsulated in a poly-acryl-nitril membrane for implantation into the rat striatum. Afterward, the host immune reaction to the membrane without encapsulated cells and the biological action of IL-6-producing capsules was evaluated. Animals with an implanted membrane without cells showed a moderate astrocytosis 5 days after theoperation. Furthermore, microglia and T-cells could be detected and after 30 days the astrocytosis decreased to a small layer around the membrane. Incomparison to the control group, which received a sham operation, our results demonstrate that the response of glial cells is caused by the mechanical damage of the surgical procedure itself rather than due to the introducedmembrane material. In contrast, we found a massive proliferation and activation of astrocytes and microglia after 10 days by IL-6-secreting capsules,indicating that IL-6 is involved in the induction of gliosis. Control animals that received encapsulated mock-transfected COS-7 cells showed only a weak response. These data point to an involvement of IL-6 in the proliferation and activation of glial cells as seen in neurodegenerative disorders. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:17:19