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Titolo:
Differences in activation of G2/M checkpoint in keratinocytes after genotoxic stress induced by hydrogen peroxide and ultraviolet a radiation
Autore:
Thorn, T; Gniadecki, R; Petersen, AB; Vicanova, J; Wulf, HC;
Indirizzi:
Univ Copenhagen, Bispebjerg Hosp, Dept Dermatol, Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark , Dept Dermatol, Copenhagen, Denmark
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 4, volume: 35, anno: 2001,
pagine: 405 - 416
SICI:
1071-5762(2001)35:4<405:DIAOGC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE ARREST; LASER-SCANNING CYTOMETRY; P53 GENE-MUTATIONS; DNA STRAND BREAKS; HUMAN-LYMPHOCYTES; COMET ASSAY; G(1) ARREST; S-PHASE; DAMAGE; IRRADIATION;
Keywords:
ultraviolet radiation; hydrogen peroxide; keratinocytes; DNA damage; cell cycle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Gniadecki, R Univ Copenhagen, Bispebjerg Hosp, Dept Dermatol, Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark ol, Copenhagen, Denmark
Citazione:
T. Thorn et al., "Differences in activation of G2/M checkpoint in keratinocytes after genotoxic stress induced by hydrogen peroxide and ultraviolet a radiation", FREE RAD RE, 35(4), 2001, pp. 405-416

Abstract

Long-wave ultraviolet radiation (UVA) may cause extensive DNA damage via reactive oxygen species (ROS). In this study we examined whether UVA- and H2O2-mediated DNA damage have equivalent effects on the induction of G2/M phase checkpoint and cell cycle progression in a transformed keratinocyte cellline HaCaT. By employing single cell gel electrophoresis (comet assay) we determined the equipotent doses of UVA and H2O2 with respect to the induction of alkali-labile sites (an indicator of oxidative DNA decay). However, in contrast to H2O2 which caused a pronounced G2/M cell cycle arrest 24 h after treatment, UVA irradiation did not affect cell cycle progression. Increasing UVA doses up to 150 kJ/m(2) did not affect cell cycle and proliferation whereas increasing H2O2 concentrations caused a cell cycle block or celldeath. Cytometric analysis revealed. that G2/M cell cycle arrest took place beyond the cyclin B1 restriction point. We conclude that the DNA damage induced by UVA is easily repaired and does not perturb cell growth, whereas the H2O2-induced damage leads ultimately to cell cycle arrest or cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:15:16