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Titolo:
Biochemical and morphological changes during development of sugar cataractin Otsuka Long-Evans Tokushima fatty (OLETF) rat
Autore:
Kubo, E; Maekawa, K; Tanimoto, T; Fujisawa, S; Akagi, Y;
Indirizzi:
Fukui Med Univ, Dept Ophthalmol, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 Dept Ophthalmol, Fukui 9101193, Japan Natl Inst Hlth Sci, Osaka Branch, Osaka 540, Japan Natl Inst Hlth Sci Osaka Japan 540 h Sci, Osaka Branch, Osaka 540, Japan
Titolo Testata:
EXPERIMENTAL EYE RESEARCH
fascicolo: 3, volume: 73, anno: 2001,
pagine: 375 - 381
SICI:
0014-4835(200109)73:3<375:BAMCDD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALDOSE REDUCTASE GENE; DEPENDENT DIABETES-MELLITUS; OSMOTIC RESPONSE ELEMENT; STRESS; LEVEL; MODEL; CELLS;
Keywords:
Otsuka Long-Evans Tokushima Fatty (OLETF) rat; sugar cataract; sorbitol accumulation; polyol pathway; aldose reductase; sorbitol dehydrogenase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Akagi, Y Fukui Med Univ, Dept Ophthalmol, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 halmol, Fukui 9101193, Japan
Citazione:
E. Kubo et al., "Biochemical and morphological changes during development of sugar cataractin Otsuka Long-Evans Tokushima fatty (OLETF) rat", EXP EYE RES, 73(3), 2001, pp. 375-381

Abstract

The relationship between the polyol pathway and sugar cataracts has been studied extensively using streptozotocin-induced diabetic rats and galactosefed rats as animal models for insulin-dependent diabetes mellitus (IDDM). In these models, sugar cataracts progress quickly, leading to rapid lenticular polyol accumulation in the early stages of cataract formation, In 1992,a new animal model of non-insulin-dependent diabetes mellitus (NIDDM), theOtsuka Long-Evans Tokushima Fatty (OLETF) rat, was established. In the present study, we examined both biochemical and morphological changes in the lenses of the OLETF rats to determine whether these changes reflect those associated with diabetic cataract formation and to clarify their relationshipwith the polyol pathway. For the biochemical analysis, we measured the enzyme activity of aldose reductase (AR) and sorbitol dehydrogenase (SDH) and the sorbitol levels using 20, 40 and 60 week old OLETF or control Long-Evans Tokushima Otsuka (LETO) rats, Enzyme activities of AR and SDH, which werelower in 20 week old OLETF rats than in LETO rats, were increased in 60 week old OLETF rats. The lenticular sorbitol level of the OLETF rats was similar to the control level at 20 weeks of age, but it was markedly increased at 40 weeks of age, and slightly decreased at 60 weeks of age compared withrats at 40 weeks but not compared with controls. Slight lens fiber swelling was observed in the anterior and/or posterior subcapsular regions of 40 week old OLETF rats, accompanying elevated sorbitol level and slightly increased SDH activity in the lens. Swelling and liquefaction of lens fibers were observed in the subcapsular and supranuclear region of 60 week old OLETF rats, as well as decreased lenticular sorbitol, and markedly increased SDH activity compared with rats at 40 weeks. AR activity was also increased causing the elevation of sorbitol in lenses of OLETF rats during the early stages of cataract formation. Despite differences in the etiology of diabetes mellitus, the strain of rat and the rate of disease progression in the OLETF rat model compared with other diabetic models, the present results support the theory that the polyol pathway via AR is a factor in the development of sugar cataracts, (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:27:05