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Titolo:
Invertebrate connectin spans as much as 3.5 mu m in the giant sarcomeres of crayfish claw muscle
Autore:
Fukuzawa, A; Shimamura, J; Takemori, S; Kanzawa, N; Yamaguchi, M; Sun, P; Maruyama, K; Kimura, S;
Indirizzi:
Chiba Univ, Fac Sci, Dept Biol, Chiba 2638522, Japan Chiba Univ Chiba Japan 2638522 Fac Sci, Dept Biol, Chiba 2638522, Japan Jikei Univ, Sch Med, Dept Physiol, Tokyo 1058461, Japan Jikei Univ TokyoJapan 1058461 h Med, Dept Physiol, Tokyo 1058461, Japan Sophia Univ, Fac Sci & Technol, Dept Chem, Tokyo 1028554, Japan Sophia Univ Tokyo Japan 1028554 Technol, Dept Chem, Tokyo 1028554, Japan Natl Ctr Univ Entrance Examinat, Tokyo 1538501, Japan Natl Ctr Univ Entrance Examinat Tokyo Japan 1538501 Tokyo 1538501, Japan
Titolo Testata:
EMBO JOURNAL
fascicolo: 17, volume: 20, anno: 2001,
pagine: 4826 - 4835
SICI:
0261-4189(20010903)20:17<4826:ICSAMA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT FLIGHT-MUSCLE; SKELETAL-MUSCLE; STRIATED-MUSCLE; ELASTIC PROTEIN; D-TITIN; Z-DISC; KETTIN; DROSOPHILA; LOCALIZATION; EXPRESSION;
Keywords:
connectin; D-titin; elasticity; kettin; titin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kimura, S Chiba Univ, Fac Sci, Dept Biol, Chiba 2638522, Japan Chiba UnivChiba Japan 2638522 Dept Biol, Chiba 2638522, Japan
Citazione:
A. Fukuzawa et al., "Invertebrate connectin spans as much as 3.5 mu m in the giant sarcomeres of crayfish claw muscle", EMBO J, 20(17), 2001, pp. 4826-4835

Abstract

In crayfish claw closer muscle, the giant sarcomeres are 8.3 mum long at rest, four times longer than vertebrate striated muscle sarcomeres, and theyare extensible up to 13 mum upon stretch. Invertebrate connectin (I-connectin) is an elastic protein which holds the A band at the center of the sarcomere. The entire sequence of crayfish I-connectin was predicted from cDNA sequences of 53 424 bp (17 352 residues; 1960 kDa). Crayfish I-connectin contains two novel 68- and 71-residue repeats, and also two PEVK domains and one kettin region. Kettin is a small isoform of I-connectin. Immunoblot tests using antibody to the 68-residue repeats revealed the presence of I-connectin also in long sarcomeres of insect leg muscle and barnacle ventral muscle. Immunofluorescence microscopy demonstrated that the two repeats, the long spacer and the two PEVK domains contribute to sarcomere extension. These regions rich in charged amino acids, occupying 63% of the crayfish I-connectin molecule, may allow a span of a 3.5 mum distance as a new class of composite spring.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:02:18