Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RNA interference of a trypanosome topoisomerase II causes progressive lossof mitochondrial DNA
Autore:
Wang, ZF; Englund, PT;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA JohnsHopkins Univ Baltimore MD USA 21205 l Chem, Baltimore, MD 21205 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 17, volume: 20, anno: 2001,
pagine: 4674 - 4683
SICI:
0261-4189(20010903)20:17<4674:RIOATT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; KINETOPLAST DNA; CRITHIDIA-FASCICULATA; GENETIC INTERFERENCE; MINICIRCLE DNA; MESSENGER-RNA; 2 SITES; REPLICATION; BRUCEI; NETWORKS;
Keywords:
kinetoplast DNA; minicircle; RNAi; topoisomerase II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Englund, PT Johns Hopkins Univ, Sch Med, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD21205 USA Johns Hopkins Univ 725 N Wolfe St Baltimore MD USA 21205 5 USA
Citazione:
Z.F. Wang e P.T. Englund, "RNA interference of a trypanosome topoisomerase II causes progressive lossof mitochondrial DNA", EMBO J, 20(17), 2001, pp. 4674-4683

Abstract

We studied the function of a Trypanosoma brucei topoisomerase II using RNAinterference (RNAi). Expression of a topoisomerase II double-stranded RNA as a stem-loop caused specific degradation of mRNA followed by loss of protein. After 6 days of RNAi, the parasites' growth rate declined and the cells subsequently died. The most striking phenotype upon induction of RNAi wasthe loss of kinetoplast DNA (kDNA), the cell's catenated mitochondrial DNAnetwork. The loss of kDNA was preceded by gradual shrinkage of the networkand accumulation of gapped free minicircle replication intermediates. These facts, together with the localization of the enzyme in two antipodal sites flanking the kDNA, show that a function of this topoisomerase II is to attach free minicircles to the network periphery following their replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:10:35