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Titolo:
Mapping of quantitative trait locus related to submergence tolerance in rice with aid of chromosome walking
Autore:
Kamolsukyunyong, W; Ruanjaichon, V; Siangliw, M; Kawasaki, S; Sasaki, T; Vanavichit, A; Tragoonrung, S;
Indirizzi:
Kasetsart Univ, Natl Ctr Genet Engn & Biotechnol, Nakorn Pathom 73140, Thailand Kasetsart Univ Nakorn Pathom Thailand 73140 akorn Pathom 73140, Thailand Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan Natl Inst Agrobiol Resource, Rice Genome Res Program, Tsukuba, Ibaraki 305, Japan Natl Inst Agrobiol Resource Tsukuba Ibaraki Japan 305 Ibaraki 305, Japan Kasetsart Univ, Dept Agron, Nakorn Pathom 73140, Thailand Kasetsart Univ Nakorn Pathom Thailand 73140 akorn Pathom 73140, Thailand
Titolo Testata:
DNA RESEARCH
fascicolo: 4, volume: 8, anno: 2001,
pagine: 163 - 171
SICI:
1340-2838(20010831)8:4<163:MOQTLR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST ARTIFICIAL CHROMOSOMES; MAP-BASED CLONING; DISEASE RESISTANCE GENE; YAC CLONES; MOLECULAR MARKERS; AFLP MARKERS; MODEL SYSTEM; BAC LIBRARY; LINKAGE MAP; CROP PLANTS;
Keywords:
rice (Oryza sativa L.); submergence tolerance; positional cloning; physical mapping; bacterial artificial chromosome (BAC); yeast artificial chromosome (YAC);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kamolsukyunyong, W Kasetsart Univ, Natl Ctr Genet Engn & Biotechnol, POB 7, Nakorn Pathom 73140, Thailand Kasetsart Univ POB 7 Nakorn Pathom Thailand 73140 land
Citazione:
W. Kamolsukyunyong et al., "Mapping of quantitative trait locus related to submergence tolerance in rice with aid of chromosome walking", DNA RES, 8(4), 2001, pp. 163-171

Abstract

The major QTL for submergence tolerance was locate in the 5.9 cM interval between flanking RFLP markers. To narrow down this region, a physical map was constructed using YAC and BAC clones. A 400-kb YAC was identified in this region and later its end fragments were used to screen a rice BAC library. Through chromosome walking, 24 positive BAC clones formed two contigs around linked-RFLP markers, R1164 and RZ698. Using one YAC end, six BAC ends and three RFLP markers, a fine-scale map was constructed of the 6.8-cM interval of S10709-RZ698 on rice chromosome 9. The submergence tolerance and related trait were located in a small, well-defined region around BAC-end marker 180D1R and RFLP marker R1164. The physical-to-map distance ratio in thisregion is as small as 172.5 kb/cM, showing that this region is a hot spot for recombination in the rice genome.

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Documento generato il 11/07/20 alle ore 09:27:08