Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Genome analysis of Agrobacterium tumefaciens: Construction of physical maps for linear and circular chromosomal DNAs, determination of copy number ratio and mapping of chromosomal virulence genes
Autore:
Suzuki, K; Iwata, K; Yoshida, K;
Indirizzi:
Hiroshima Univ, Grad Sch Sci, Dept Sci Biol, Div Plant Biol,Lab Plant Mol Biol & Bioinformat, Higashihiroshima 7398526, Japan Hiroshima Univ Higashihiroshima Japan 7398526 hihiroshima 7398526, Japan
Titolo Testata:
DNA RESEARCH
fascicolo: 4, volume: 8, anno: 2001,
pagine: 141 - 152
SICI:
1340-2838(20010831)8:4<141:GAOATC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TI PLASMID; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; BRUCELLA-MELITENSIS; VIBRIO-CHOLERAE; ORGANIZATION; SEQUENCE; TRANSDUCTION; CONJUGATION; ATTACHMENT;
Keywords:
bacterial chromosome; copy number; genome organization; linear and circular genomes; pathogenesis genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshida, K Hiroshima Univ, Grad Sch Sci, Dept Sci Biol, Div Plant Biol,LabPlant Mol Biol & Bioinformat, Higashihiroshima 7398526, Japan Hiroshima Univ Higashihiroshima Japan 7398526 7398526, Japan
Citazione:
K. Suzuki et al., "Genome analysis of Agrobacterium tumefaciens: Construction of physical maps for linear and circular chromosomal DNAs, determination of copy number ratio and mapping of chromosomal virulence genes", DNA RES, 8(4), 2001, pp. 141-152

Abstract

The phytopathogenic bacterium Agrobacterium tumefaciens is unique in that it possesses both linear and circular DNA chromosomes in addition to a plant-tumor-inducing (Ti) plasmid. We analyzed the two chromosomal DNA molecules in strain MAFF301001, whose Ti plasmid has already been sequenced Completely. Physical maps of the chromosomal DNAs were constructed by Southern hybridization experiments using Pme I and Swa I fragments and short fragments bridging the Swa I fragments with special care to avoid any missing fragment. Hybridization with 16S rDNA probe showed one rDNA locus on the linear chromosome and two loci on the circular chromosome. For this bacterium to be pathogenic, not only Ti plasmid but also chromosomal genes are required. The chromosomal virulence (chv) genes (chvA, chvB, chvD, chvE, chvG, chvH, and chvI) and the chromosomal genes affecting the virulence [acvB, pgm(exoC),glgP, miaA, and ros] were successfully mapped onto 5 different regions in the chromosomal physical maps. These chv genes and the chromosomal genes affecting the virulence other than pgm and glgP were found on the circular chromosome, whereas the pgm and glgP genes were located on the linear chromosome. In contrast to the large terminal inverted repeats of Streptomyces linear chromosomal DNA, no hybridization signal was detected between left and right terminal fragments of the linear A. tumefaciens chromosome. Quantitative analysis of DNA fragments indicated that the copy numbers of the two chromosomal DNAs and the Ti plasmid are identical.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 04:10:50