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Titolo:
Inducible nitric oxide synthase expression in Leishmania-infected dog macrophages
Autore:
Sisto, M; Brandonisio, O; Panaro, MA; Acquafredda, A; Leogrande, D; Fasanella, A; Trotta, T; Fumarola, L; Mitolo, V;
Indirizzi:
Univ Bari, Dipartimento Anat Umana & Istol, Bari, Italy Univ Bari Bari Italy Bari, Dipartimento Anat Umana & Istol, Bari, Italy Univ Bari, Dipartimento Clin Med Immunol & Malattie Infett, Sez Microbiol & Immunol, Bari, Italy Univ Bari Bari Italy attie Infett, Sez Microbiol & Immunol, Bari, Italy Ist Zooprofilatt Sperimentale Puglia & Basilicata, Foggia, Italy Ist Zooprofilatt Sperimentale Puglia & Basilicata Foggia Italy a, Italy
Titolo Testata:
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
fascicolo: 4, volume: 24, anno: 2001,
pagine: 247 - 254
SICI:
0147-9571(200110)24:4<247:INOSEI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED MACROPHAGES; MOUSE MACROPHAGES; INTERFERON-GAMMA; INFANTUM; LIPOPOLYSACCHARIDE; DONOVANI; MULTIPLICATION; AMASTIGOTES; LYMPHOKINE;
Keywords:
dog; nitric oxide synthase (NOS); Leishmania;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Panaro, MA Univ Bari, Dipartimento Anat Umana & Istol, Bari, Italy Univ Bari Bari Italy timento Anat Umana & Istol, Bari, Italy
Citazione:
M. Sisto et al., "Inducible nitric oxide synthase expression in Leishmania-infected dog macrophages", COMPAR IMM, 24(4), 2001, pp. 247-254

Abstract

Nitric oxide (NO) production by the inducible NO synthase (iNOS or NOS2) represents one of the main microbicidal mechanisms of murine macrophages, but its role in other animal models is poorly investigated. Therefore, the aim of this work was to evaluate NOS2 expression in dog macrophages infected with Leishmania infantum. Macrophages obtained from peripheral blood of healthy dogs were activated with recombinant human interferon (rhIFN)-gamma and bacterial lipopolysaccharide (LPS) and then infected with L. infantum promastigotes, zymodeme MON1. For the immunofluorescence assay fixed macrophages were incubated with polyclonal rabbit anti-NOS2 and then with rhodamine F(ab ')(2) goat anti-rabbit IgG. For immunoblotting, cell lysates were submitted to SDS-PAGE and blots were incubated with polyclonal rabbit anti-NOS2and then with horseradish peroxidase-conjugated goat anti-rabbit IgG. Results demonstrated that L. infantum-infected cells, after stimulation with rhIFN-gamma and LPS, displayed high levels of fluorescence for the NOS2 in their cytoplasm, unlike unstimulated uninfected macrophages. In western blotting, polyclonal anti-NOS2 reacted specifically with a protein band corresponding to 130 kDa. The signal produced in Leishmania-infected cells stimulated with rhIFN-gamma and LPS was higher than that produced in Leishmania-infected unstimulated cells. No band was detected in cellular lysates from uninfected unstimulated cells. These results indicate that dog macrophages canexpress NOS2, and suggest a role for IFN-gamma and LPS in NOS2 induction also in this animal model. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 30/03/20 alle ore 13:28:11